ArticleProteomics. Clinical applications2018
"One-Pot" Sample Processing Method for Proteome-Wide Analysis of Microbial Cells and Spores.
Article in Proteomics. Clinical applications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- Germination ofJournal of bacteriology · 2025Article
- Article
- Ionic liquid-assisted sample preparation mediates sensitive proteomic analysis of Bacillus subtilis spores.Scientific reports · 2024Article
- Integrative Metabolomics and Proteomics Allow the Global Intracellular Characterization ofJournal of proteome research · 2024Article
- Expression of the 2Duf protein in wild-type Bacillus subtilis spores stabilizes inner membrane proteins and increases spore resistance to wet heat and hydrogen peroxide.Journal of applied microbiology · 2023Article
- Identification and characterization of new proteins crucial for bacterial spore resistance and germination.Frontiers in microbiology · 2023Article
- Time-Resolved Proteomics of Germinating Spores ofInternational journal of molecular sciences · 2022Article
- Changes in the Spore Proteome ofMicroorganisms · 2022Article
- Moderate High-Pressure Superdormancy inApplied and environmental microbiology · 2022Article
- Dynamics of Germinosome Formation and FRET-Based Analysis of Interactions between GerD and Germinant Receptor Subunits inInternational journal of molecular sciences · 2021Article
- High Resolution Analysis of Proteome Dynamics duringInternational journal of molecular sciences · 2021Article
- Levels and Characteristics of mRNAs in Spores of Firmicute Species.Journal of bacteriology · 2021Article
- Identification of Native Cross-Links inJournal of proteome research · 2021Article
- Analysis of disulphide bond linkage between CoA and protein cysteine thiols during sporulation and in spores of Bacillus species.FEMS microbiology letters · 2020Article
- Article
- Integrative Analysis of Proteome and Transcriptome Dynamics during Bacillus subtilis Spore Revival.mSphere · 2020Article
- Artificial Sporulation Induction (ASI) byInternational journal of molecular sciences · 2020Article
- Bacterial Spore mRNA - What's Up With That?Frontiers in microbiology · 2020Review
- Vegetative Cell and Spore Proteomes ofJournal of proteome research · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
purposeBacterial endospores, the transmissible forms of pathogenic bacilli and clostridia, are heterogeneous multilayered structures composed of proteins. These proteins protect the spores against a variety of stresses, thus helping spore survival, and assist in germination, by interacting with the environment to form vegetative cells. Owing to the complexity, insolubility, and dynamic nature of spore proteins, it has been difficult to obtain their comprehensive protein profiles. EXPERIMENTAL
designThe intact spores of Bacillus subtilis, Bacillus cereus, and Peptoclostridium difficile and their vegetative counterparts were disrupted by bead beating in 6 m urea under reductive conditions. The heterogeneous mixture was then double digested with LysC and trypsin. Next, the peptide mixture was pre-fractionated with zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC) followed by reverse-phase LC-FT-MS analysis of the fractions.
results"One-pot" method is a simple, robust method that yields identification of >1000 proteins with high confidence, across all spore layers from B. subtilis, B. cereus, and P. difficile. CONCLUSIONS AND MEDICAL RELEVANCE: This method can be employed for proteome-wide analysis of non-spore-forming as well as spore-forming pathogens. Analysis of spore protein profile will help to understand the sporulation and germination processes and to distinguish immunogenic protein markers.
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Registered trials
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