ArticlePlant methods2018
A simple and rapid method for preparing the whole section of starchy seed to investigate the morphology and distribution of starch in different regions of seed.
Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- NAC25 transcription factor regulates the degeneration of cytoplasmic membrane integrity and starch biosynthesis in rice endosperm through interacting with MADS29.Frontiers in plant science · 2025Article
- SUBSTANDARD STARCH GRAIN7 regulates starch grain size and endosperm development in rice.Plant biotechnology journal · 2024Article
- Some Aspects of the Physiology of theLife (Basel, Switzerland) · 2023Article
- Active-type starch synthase (SS) IIa from indica rice partially complements the sugary-1 phenotype in japonica rice endosperm.Plant molecular biology · 2022Article
- Starch and Glycogen Analyses: Methods and Techniques.Biomolecules · 2020Review
- The defective effect of starch branching enzyme IIb from weak to strong induces the formation of biphasic starch granules in amylose-extender maize endosperm.Plant molecular biology · 2020Article
- Comprehensive comparison and applications of different sections in investigating the microstructure and histochemistry of cereal kernels.Plant methods · 2020Review
- Imaging Amyloplasts in the Developing Endosperm of Barley and Rice.Scientific reports · 2019Article
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Authors and funding
4 authors.
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Abstract
backgroundStorage starch in starchy seed influences the seed weight and texture, and determines its applications in food and nonfood industries. Starch granules from different plant sources have significantly different shapes and sizes, and even more the difference exists in the different regions of the same tissue. Therefore, it is very important to in situ investigate the morphology and distribution of starch in the whole seed. However, a simple and rapid method is deficient to prepare the whole section of starchy seed for investigating the morphology and distribution of starch in the whole seeds for a large number of samples.
resultsA simple and rapid method was established to prepare the whole section of starchy seed, especially for floury seed, in this study. The whole seeds of translucent and chalky rice, vitreous and floury maize, and normal barley and wheat were sectioned successfully using the newly established method. The iodine-stained section clearly exhibited the shapes and size of starch granules in different regions of seed. The starch granules with different morphologies and iodine-staining colors existed regionally in the seeds of high-amylose rice and maize. The sections of lotus and kidney bean seeds also showed the feasibility of this method for starchy non-cereal seeds.
conclusionThe simple and rapid method was proven effective for preparing the whole sections of starchy seeds. The whole section of seed could be used to investigate the morphology and distribution of starch granules in different regions of the whole seed. The method was especially suitable for large sample numbers to investigate the starch morphology in short time.
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