ArticleNature structural & molecular biology2018
Screening, large-scale production and structure-based classification of cystine-dense peptides.
Article in Nature structural & molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
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- Review
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- Synthesis and preclinical evaluation of aEuropean journal of nuclear medicine and molecular imaging · 2025Article
- Spatial charge-hydrophobicity configuration modulates cationic peptide transport in cartilage.Biophysical journal · 2025Article
- Scorpion Venom as a Source of Cancer Drugs: A Comprehensive Proteomic Analysis and Therapeutic Potential.International journal of molecular sciences · 2025Review
- Embracing Complexity: Peptides as Tunable Scaffolds in the Construction of Discrete Supramolecular Systems.Angewandte Chemie (International ed. in English) · 2025Review
- Spatiotemporal Control Over Protein Release from Artificial Cells via a Light-Activatable Protease.Advanced biology · 2025Article
- An improved variant of tobacco etch virus (TEV) protease that does not need reducing agents.Protein science : a publication of the Protein Society · 2025Article
- The First KToxins · 2025Article
- Enzyme-substrate co-display on yeast empowers engineering of tobacco etch virus protease activity.Protein engineering, design & selection : PEDS · 2025Article
- Integrated machine learning and single-cell analysis reveal the prognostic and therapeutic potential of SUMOylation-related genes in ovarian cancer.Frontiers in immunology · 2025Article
- Cnidarian toxins: omics approaches and recombinant proteins.The journal of venomous animals and toxins including tropical diseases · 2025Article
- ω-Grammotoxin-SIA inhibits voltage-gated Na+ channel currents.The Journal of general physiology · 2024Article
- Systematic Druggable Genome-Wide Mendelian Randomization Identifies Therapeutic Targets for Functional Outcome After Ischemic Stroke.Journal of the American Heart Association · 2024Article
- Modulation of Apoptotic, Cell Cycle, DNA Repair, and Senescence Pathways by Marine Algae Peptides in Cancer Therapy.Marine drugs · 2024Review
- Molecular Display of the Animal Meta-Venome for Discovery of Novel Therapeutic Peptides.bioRxiv : the preprint server for biology · 2024Article
- Improving Protein Expression, Stability, and Function with ProteinMPNN.Journal of the American Chemical Society · 2024Article
- Siderocalin fusion proteins enable a newRSC chemical biology · 2023Article
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Authors and funding
18 authors.
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Abstract
Peptides folded through interwoven disulfides display extreme biochemical properties and unique medicinal potential. However, their exploitation has been hampered by the limited amounts isolatable from natural sources and the expense of chemical synthesis. We developed reliable biological methods for high-throughput expression, screening and large-scale production of these peptides: 46 were successfully produced in multimilligram quantities, and >600 more were deemed expressible through stringent screening criteria. Many showed extreme resistance to temperature, proteolysis and/or reduction, and all displayed inhibitory activity against at least 1 of 20 ion channels tested, thus confirming their biological functionality. Crystal structures of 12 confirmed proper cystine topology and the utility of crystallography to study these molecules but also highlighted the need for rational classification. Previous categorization attempts have focused on limited subsets featuring distinct motifs. Here we present a global definition, classification and analysis of >700 structures of cystine-dense peptides, providing a unifying framework for these molecules.
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