ArticleNature chemical biology2018
A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters.
Article in Nature chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 201 papers.
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Who cites it
201 citing papers in PubMed, 345 citations in OpenAlex.
- Voltage imaging: A practical guide.Neuron · 2026Review
- Methods, Trade-offs, and Opportunities in High-speed Optical Microscopy for Neural Voltage imaging.Nature photonics · 2026Article
- Voltage imaging of neurons distributed across entire brains of larval zebrafish.Nature methods · 2026Article
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
- Rhobot-Screen: an integrated robotic platform for functional screening of rhodopsin variants.BMC biology · 2026Article
- Plasmonic Enhancement of Fluorescence and Protein Dynamics in Living Mammalian Cells.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- Three-dimensional voltage imaging in live larval zebrafish brains using fully genetically encoded voltage indicator.Scientific reports · 2026Article
- Beyond Membrane Potential: Exploiting Signal Complexity in Genetically Encoded Voltage Indicators.Sensors (Basel, Switzerland) · 2026Article
- A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo.bioRxiv : the preprint server for biology · 2026Article
- Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Cancer: A bioelectric disease?Clinical and translational medicine · 2026Review
- Voltage imaging in zebrafish using high-speed light-sheet microscopy.Neurophotonics · 2026Review
- Article
- Live-cell physiology in human brain tissue culture-the potential, the challenges, and the lessons learned.Frontiers in cellular neuroscience · 2026Review
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells.bioRxiv : the preprint server for biology · 2025Article
- Rigor & Reproducibility: pH Adjustments of Papain with L-Cysteine Dissociation Solutions and Cell Media Using Phenol Red Spectrophotometry.Biosensors · 2025Article
- Real-time self-supervised denoising for high-speed fluorescence neural imaging.Nature communications · 2025Article
- Semirational Protein Engineering Yields Archaerhodopsin-3-Based Fluorescent Genetically Encoded Voltage Indicators with Enhanced Brightness and Red Shifted Absorption Bands.Chem & bio engineering · 2025Article
- Lamin B1 and LAP2β resist cytoskeletal force to maintain lamin A/C meshwork organization and preserve nuclear integrity.Molecular biology of the cell · 2025Article
141 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
27 authors at 10 institutions in 3 countries.
Funding
Abstract
We developed a new way to engineer complex proteins toward multidimensional specifications using a simple, yet scalable, directed evolution strategy. By robotically picking mammalian cells that were identified, under a microscope, as expressing proteins that simultaneously exhibit several specific properties, we can screen hundreds of thousands of proteins in a library in just a few hours, evaluating each along multiple performance axes. To demonstrate the power of this approach, we created a genetically encoded fluorescent voltage indicator, simultaneously optimizing its brightness and membrane localization using our microscopy-guided cell-picking strategy. We produced the high-performance opsin-based fluorescent voltage reporter Archon1 and demonstrated its utility by imaging spiking and millivolt-scale subthreshold and synaptic activity in acute mouse brain slices and in larval zebrafish in vivo. We also measured postsynaptic responses downstream of optogenetically controlled neurons in C. elegans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.