ArticleScientific reports2018
Understanding the high L-valine production in Corynebacterium glutamicum VWB-1 using transcriptomics and proteomics.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 48 citations in OpenAlex.
- Screening of high glucose tolerant Escherichia coli for L-valine fermentation by autonomous evolutionary mutation.Applied microbiology and biotechnology · 2025Article
- Branched-chain amino acids: physico-chemical properties, industrial synthesis and role in signaling, metabolism and energy production.Amino acids · 2024Review
- Integrative transcriptome and proteome revealed high-yielding mechanisms of epsilon-poly-L-lysine byFrontiers in microbiology · 2023Article
- Metabolic engineering of Corynebacterium glutamicum for producing branched chain amino acids.Microbial cell factories · 2021Review
- L-valine production in Corynebacterium glutamicum based on systematic metabolic engineering: progress and prospects.Amino acids · 2021Review
- Engineering of microbial cells for L-valine production: challenges and opportunities.Microbial cell factories · 2021Review
- Metabolic fluxes for nutritional flexibility of Mycobacterium tuberculosis.Molecular systems biology · 2021Article
- Genomics Characterization of an EngineeredFrontiers in bioengineering and biotechnology · 2021Article
- The Characterization of Two-Component System PmrA/PmrB inFrontiers in microbiology · 2020Article
- Recent Advances of L-ornithine Biosynthesis in Metabolically EngineeredFrontiers in bioengineering and biotechnology · 2019Review
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Authors and funding
4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Toward the elucidation of the advanced mechanism of L-valine production by Corynebacterium glutamicum, a highly developed industrial strain VWB-1 was analyzed, employing the combination of transcriptomics and proteomics methods. The transcriptional level of 1155 genes and expression abundance of 96 proteins were changed significantly by the transcriptome and proteome comparison of VWB-1 and ATCC 13869. It was indicated that the key genes involved in the biosynthesis of L-valine, ilvBN, ilvC, ilvD, ilvE were up-regulated in VWB-1, which together made prominent contributions in improving the carbon flow towards L-valine. The L-leucine and L-isoleucine synthesis ability were weakened according to the down-regulation of leuB and ilvA. The up-regulation of the branched chain amino acid transporter genes brnFE promoted the L-valine secretion capability of VWB-1. The NADPH and ATP generation ability of VWB-1 were strengthened through the up-regulation of the genes involved in phosphate pentose pathway and TCA pathway. Pyruvate accumulation was achieved through the weakening of the L-lactate, acetate and L-alanine pathways. The up-regulation of the genes coding for elongation factors and ribosomal proteins were beneficial for L-valine synthesis in C. glutamicum. All information acquired were useful for the genome breeding of better industrial L-valine producing strains.
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