Evidence map›Paper›PMID 29482510›Full record

ArticleBMC ophthalmology2018

P66Shc expression in diabetic rat retina.

Ming-Hui Zhao, Jianyan Hu, Shufeng Li, Qiang Wu, Peirong Lu

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC ophthalmology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.4field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Ming-Hui ZhaoDepartment of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.
Jianyan HuDepartment of Ophthalmology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
Shufeng LiDepartment of Ophthalmology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
Qiang WuDepartment of Ophthalmology, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, 200233, China.
Peirong LuDepartment of Ophthalmology, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China. lupeirongsz@126.com.ORCID http://orcid.org/0000-0001-7534-4930
Shanghai Sixth People's Hospital · CNSoochow University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundP66Shc is partially localised within the mitochondrial fraction. It is primarily related to the generation of mitochondrial reactive oxygen species and apoptosis. Based on previous studies, we hypothesize that in the retina, p66Shc may exist and affect the development of diabetic retinopathy. The purpose of this study was to investigate p66Shc expression in retinal in streptozotocin-induced diabetic (SD) rats, which may provide a pathway to study the pathogenesis of diabetic retinopathy.

methodsReverse transcription-polymerase chain reaction (RT-PCR) and western blot were used to detect retinal p66Shc mRNA and protein expression in SD rats, respectively. Immunohistochemical staining was applied to detect the location of rat retinal p66Shc expression. TUNEL assay was applied to detect the number of apoptotic cells.

resultsP66Shc expression was found in the retina of normal and diabetic rats, and the level of mRNA and protein expression increased with the progression of diabetes mellitus (DM). P66Shc expression was mainly located in the retinal ganglion cell layer and inner nuclear layer. Compared with the normal group, retinal cell tissue apoptosis rate in the D12w group was significantly increased.

conclusionRat retinal p66Shc expression was mainly in the ganglion cell layer and inner nuclear layer. As the degree of DM progressed, p66Shc expression gradually increased, and the number of apoptotic cells also increased.

Indexed as

AnimalsBlotting, WesternDiabetes Mellitus, ExperimentalDiabetic RetinopathyImmunohistochemistryIn Situ Nick-End LabelingMaleRatsRats, Sprague-DawleyReal-Time Polymerase Chain ReactionRetinaRetinal Ganglion CellsSrc Homology 2 Domain-Containing, Transforming Protein 1Shc1 protein, ratSrc Homology 2 Domain-Containing, Transforming Protein 1ApoptosisDiabetic retinopathyOxidative stressp66Shc, reactive oxygen species

Identifiers

PMID29482510
PMCPMC6389069
OpenAlexW2796932095

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.