ArticleNucleic acids research2018
N4-cytosine DNA methylation regulates transcription and pathogenesis in Helicobacter pylori.
Article in Nucleic acids research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 50 papers.
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50 citing papers in PubMed, 76 citations in OpenAlex.
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- A Modified Methyl Transferase Cofactor to Selectively Silence Gene Expression in Escherichia coli.Chembiochem : a European journal of chemical biology · 2026Article
- Epigenetic regulation and antimicrobial resistance: functional roles of DNA methylation.FEMS microbiology reviews · 2026Review
- MPore: database-driven identification of active methyltransferases in prokaryotic genomes from nanopore sequencing.Bioinformatics advances · 2026Article
- The emerging role of DNA methylation in the pathogenicity of bacterial pathogens.Journal of bacteriology · 2025Review
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- Insights into Functions of Universal Stress Proteins Encoded by Genomes of Gastric Cancer PathogenPathogens (Basel, Switzerland) · 2025Article
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- Intercellular mosaic methylation in fast-growingNAR molecular medicine · 2024Article
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- Characterization of a Novel N4-Methylcytosine Restriction-Modification System inInternational journal of molecular sciences · 2024Article
- TheJournal of bacteriology · 2024Article
- DNA methylases for site-selective inhibition of type IIS restriction enzyme activity.Applied microbiology and biotechnology · 2024Article
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- Methylome evolution suggests lineage-dependent selection in the gastric pathogen Helicobacter pylori.Communications biology · 2023Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many bacterial genomes exclusively display an N4-methyl cytosine base (m4C), whose physiological significance is not yet clear. Helicobacter pylori is a carcinogenic bacterium and the leading cause of gastric cancer in humans. Helicobacter pylori strain 26695 harbors a single m4C cytosine methyltransferase, M2.HpyAII which recognizes 5' TCTTC 3' sequence and methylates the first cytosine residue. To understand the role of m4C modification, M2.hpyAII deletion strain was constructed. Deletion strain displayed lower adherence to host AGS cells and reduced potential to induce inflammation and apoptosis. M2.hpyAII gene deletion strain exhibited reduced capacity for natural transformation, which was rescued in the complemented strain carrying an active copy of M2.hpyAII gene in the genome. Genome-wide gene expression and proteomic analysis were carried out to discern the possible reasons behind the altered phenotype of the M2.hpyAII gene deletion strain. Upon the loss of m4C modification a total of 102 genes belonging to virulence, ribosome assembly and cellular components were differentially expressed. The present study adds a functional role for the presence of m4C modification in H. pylori and provides the first evidence that m4C signal acts as a global epigenetic regulator in H. pylori.
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