ArticleNature methods2018
A hybridization-chain-reaction-based method for amplifying immunosignals.
Article in Nature methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed.
- Emerging technologies in the spatial proteomics landscape.Analytical and bioanalytical chemistry · 2026Review
- CellTune: an integrative software for accurate cell classification in spatial proteomics.Nature methods · 2026Article
- Multimodal spatial omics: From data acquisition to computational integration.Patterns (New York, N.Y.) · 2026Review
- High-dimensional imaging using combinatorial channel multiplexing and deep learning.Nature biotechnology · 2026Article
- MicroRNA-triggeredNational science review · 2026Article
- The evolving landscape of spatial proteomics technologies in the AI age.Fundamental research · 2026Review
- Article
- Mimicking a Cellular Crowding Environment for Enzyme-Free Paper-Based Nucleic Acid Tests at the Point of Care.ACS sensors · 2024Article
- Multiplexed in situ protein imaging using DNA-barcoded antibodies with extended hybridization chain reactions.Nucleic acids research · 2024Article
- On-demand expansion fluorescence and photoacoustic microscopy (ExFLPAM).Photoacoustics · 2024Article
- The effect of P2X7 antagonism on subcortical spread of optogenetically-triggered cortical spreading depression and neuroinflammation.The journal of headache and pain · 2024Article
- Development of surface-enhanced Raman scattering-sensing Method by combining novel Ag@Au core/shell nanoparticle-based SERS probe with hybridization chain reaction for high-sensitive detection of hepatitis C virus nucleic acid.Analytical and bioanalytical chemistry · 2024Article
- Mimicking anbioRxiv : the preprint server for biology · 2024Article
- GelMap: intrinsic calibration and deformation mapping for expansion microscopy.Nature methods · 2023Article
- DNA-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging.Nature methods · 2023Article
- Article
- Expansion microscopy: A chemical approach for super-resolution microscopy.Current opinion in structural biology · 2023Review
- CellSighter: a neural network to classify cells in highly multiplexed images.Nature communications · 2023Article
- The emerging landscape of spatial profiling technologies.Nature reviews. Genetics · 2022Review
- Super-Resolution Vibrational Imaging Using Expansion Stimulated Raman Scattering Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunosignal hybridization chain reaction (isHCR) combines antibody-antigen interactions with hybridization chain reaction (HCR) technology, which results in amplification of immunofluorescence signals by up to two to three orders of magnitude with low background. isHCR's highly modular and easily adaptable design enables the technique to be applied broadly, and we further optimized its use in multiplexed imaging and in state-of-the-art tissue expansion and clearing techniques.
Indexed as
Identifiers
29481551What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.