ArticleConservation physiology2018
A novel method for the measurement of glucocorticoids in dermal secretions of amphibians.
Article in Conservation physiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- The adrenal stress response involves distinct dynamics of both cortisol and corticosterone in the axolotl salamander.Lab animal · 2026Article
- Combined impacts of warming and methomyl on neurophysiological and behavioral responses in Amazonian frog tadpoles.Ecotoxicology (London, England) · 2026Article
- Stress, Well-Being, and Reproduction in Wildlife.Advances in experimental medicine and biology · 2026Review
- Investigating the Temporal Effects of Thermal Stress on Corticosterone Release and Growth in Toad Tadpoles.Biology · 2025Article
- Validation of non-invasive methods for the measurement of gonadal and inter-renal steroid hormones in a desert-adapted amphibian (Conservation physiology · 2025Article
- Dermal glucocorticoids are uncoupled from stress physiology and infection.Conservation physiology · 2025Article
- Why not both? A case study in measuring cortisol and corticosterone.Integrative organismal biology (Oxford, England) · 2025Article
- Physiological state matching in a pair bonded poison frog.Royal Society open science · 2024Article
- Framework for multi-stressor physiological response evaluation in amphibian risk assessment and conservation.Frontiers in ecology and evolution · 2024Article
- Article
- Linking microbiome and stress hormone responses in wild tropical treefrogs across continuous and fragmented forests.Communications biology · 2023Article
- Current State of Conservation Physiology for Amphibians: Major Research Topics and Physiological Parameters.Animals : an open access journal from MDPI · 2023Review
- Exploring the Effect of Age on the Reproductive and Stress Physiology ofAnimals : an open access journal from MDPI · 2023Article
- Comparison of Corticosterone Concentrations in Dermal Secretions and Urine in Free-Ranging Marine Toads (Veterinary medicine international · 2023Article
- Exploring water-borne corticosterone collection as a non-invasive tool in amphibian conservation physiology: benefits, limitations and future perspectives.Conservation physiology · 2023Article
- Preliminary Investigation into Developing the Use of Swabs for Skin Cortisol Analysis for the Ocean Sunfish (Animals : an open access journal from MDPI · 2022Article
- A Review of Non-Invasive Sampling in Wildlife Disease and Health Research: What's New?Animals : an open access journal from MDPI · 2022Review
- Using dermal glucocorticoids to determine the effects of disease and environment on the critically endangered Wyoming toad.Conservation physiology · 2021Article
- Beyond Glucocorticoids: Integrating Dehydroepiandrosterone (DHEA) into Animal Welfare Research.Animals : an open access journal from MDPI · 2020Review
- Biomarker and hematological fieldwork with amphibians: is it necessary to sample all night?Environmental science and pollution research international · 2020Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Amphibians have been declining in both diversity and abundance due in large part to habitat degradation and the prevalence of emerging diseases. Although stressors can suppress the immune system, affecting an individual's health and susceptibility to pathogens, established methods for directly collecting stress hormones are not suitable for rapid field use or for use on threatened and endangered species. To overcome these challenges, we are developing an innovative method to collect and measure amphibian glucocorticoid secretions using non-invasive dermal swabs. We tested this methodology using multiple terrestrial, semi-aquatic and fully aquatic species. We swabbed the dorsal side of each animal six times and then induced a stressor of either hand-restraint, ACTH injection, or saline as a control. We then repeated swab collection immediately after the stressor and at 15, 30, 45, 60, 90 and 120 min intervals. Cortisol enzyme immunoassay detected changes in cortisol post-stressor. We also tested this methodology in the field and were successfully able to detect glucocorticoids from multiple species at varying life stages. When using in the field, capture technique should be considered since it may impact stress levels in certain species. Upon further testing, this novel method may be used to greatly increase our understanding of amphibian health especially as disease and environmental changes continue to impact fragile populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.