ArticleEpidemiology and infection2018
Canine visceral leishmaniasis diagnosis: a comparative performance of serological and molecular tests in symptomatic and asymptomatic dogs.
Article in Epidemiology and infection, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Seroprevalence and Factors Associated with Canine Leishmaniasis in Baringo County, Kenya: Implications for an Integrated One Health Surveillance.Veterinary sciences · 2026Article
- Community Drivers ofTropical medicine and infectious disease · 2026Article
- Canine leishmaniasis in the Atlantic Rainforest Biome region of Bahia, Brazil, affected by deforestation: a one health perspective.Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria · 2026Article
- Global Distribution of Canine Visceral Leishmaniasis and the Role of the Dog in the Epidemiology of the Disease.Pathogens (Basel, Switzerland) · 2024Review
- Comparative Study of Colorimetric In Situ Hybridization and Quantitative Real-Time Polymerase Chain Reaction for Diagnosis of Infection byTropical medicine and infectious disease · 2024Article
- Refinement of the rKLi8.3-Based Serodiagnostic ELISA Allows Detection of Canine Leishmaniosis in Dogs with Low Antibody Titers.Pathogens (Basel, Switzerland) · 2024Article
- Article
- Plant Expression of Hydrophobin Fused K39 Antigen for Visceral Leishmaniasis Immunodiagnosis.Frontiers in plant science · 2021Article
- Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic tests.Revista da Sociedade Brasileira de Medicina Tropical · 2021Article
- Epidemiological aspects and spatial patterns of human visceral leishmaniasis in Brazil.Parasitology · 2020Article
- Improving the serodiagnosis of canineParasite epidemiology and control · 2020Article
- How much does it cost to prevent and control visceral leishmaniasis in Brazil? Comparing different measures in dogs.PloS one · 2020Article
- Leishmania infantum-derived lipophosphoglycan as an antigen in the accurate serodiagnosis of canine leishmaniasis.PLoS neglected tropical diseases · 2019Article
- eNose analysis of volatile chemicals from dogs naturally infected with Leishmania infantum in Brazil.PLoS neglected tropical diseases · 2019Article
- Evaluation of three recombinant proteins for the development of ELISA and immunochromatographic tests for visceral leishmaniasis serodiagnosis.Memorias do Instituto Oswaldo Cruz · 2019Article
- Improving the reference standard for the diagnosis of canine visceral leishmaniasis: a challenge for current and future tests.Memorias do Instituto Oswaldo Cruz · 2019Article
- Comparative analysis of real-time PCR assays in the detection of canine visceral leishmaniasis.Parasitology research · 2018Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although serological assays have been widely used for the diagnosis of canine visceral leishmaniasis (CVL), they present different performances depending on the clinical profile of the dogs. This study evaluated the accuracy of serological tests, immunochromatographic (Dual Path Platform: DPP®) and enzyme-linked immunosorbent (ELISA EIE®), for CVL in relation to the detection of Leishmania DNA through real-time polymerase chain reaction (real-time PCR) in samples from symptomatic and asymptomatic dogs from a non-endemic area in the state of Rio Grande do Sul, Southern Brazil. Serum from 140 dogs (39 symptomatic and 101 asymptomatic) was tested by DPP and ELISA followed by real-time PCR. From a total of 140 samples evaluated, Leishmania DNA was detected by real-time PCR in 41.4% (58/140). Moreover, 67.2% of samples positive in real-time PCR were positive in both DPP and ELISA (39/58), showing moderate agreement between methods. In the symptomatic group, one sample non-reactive in both serological assays was positive in real-time PCR, whereas in the asymptomatic group, 17.8% non-reactive or undetermined samples in serological assays were positive in the molecular method. Leishmania DNA was not detected in 17.9% reactive samples by serological assays from the symptomatic group, and in 3.9% from asymptomatic dogs. Real-time PCR demonstrated greater homogeneity between symptomatic and asymptomatic groups compared with DPP and ELISA. The molecular method can help to establish the correct CVL diagnosis, particularly in asymptomatic dogs, avoiding undesirable euthanasia.
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