ArticlePlant biotechnology journal2018
Induction and recovery of copy number variation in banana through gamma irradiation and low-coverage whole-genome sequencing.
Article in Plant biotechnology journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Chromosomal deletions in banana somaclonal variants reveal negative regulators of immunity underlyingProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Genome-Wide Characterization of a Carbon Ion Beam-Induced Soybean Mutant Population Reveals Extensive Genetic Variation for Trait Improvement.International journal of molecular sciences · 2025Article
- Shaping the future of bananas: advancing genetic trait regulation and breeding in the postgenomics era.Horticulture research · 2025Article
- Identification of Induced Copy Number Variation from Low Coverage Sequence Data.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Effect of gamma irradiation on proliferation and growth of friable embryogenic callus andFrontiers in plant science · 2024Article
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- Article
- Cultivar-specific markers, mutations, and chimerisim of Cavendish banana somaclonal variants resistant to Fusarium oxysporum f. sp. cubense tropical race 4.BMC genomics · 2022Article
- Genome sequencing-based coverage analyses facilitate high-resolution detection of deletions linked to phenotypes of gamma-irradiated wheat mutants.BMC genomics · 2022Article
- Frequency and Spectrum of Mutations Induced by Gamma Rays Revealed by Phenotype Screening and Whole-Genome Re-Sequencing inInternational journal of molecular sciences · 2022Article
- FirstGigaByte (Hong Kong, China) · 2022Article
- Article
- A Novel Banana Mutant "Frontiers in plant science · 2021Article
- Comparison and Characterization of Mutations Induced by Gamma-Ray and Carbon-Ion Irradiation in Rice (G3 (Bethesda, Md.) · 2019Article
- DNA Copy Number Variations as Markers of Mutagenic Impact.International journal of molecular sciences · 2019Review
- Fragmentation of Pooled PCR Products for Highly Multiplexed TILLING.G3 (Bethesda, Md.) · 2019Article
- Screening populations for copy number variation using genotyping-by-sequencing: a proof of concept using soybean fast neutron mutants.BMC genomics · 2019Article
- Genetic Variability Induced by Gamma Rays and Preliminary Results of Low-Cost TILLING on MFrontiers in plant science · 2018Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Traditional breeding methods are hindered in bananas due to the fact that major cultivars are sterile, parthenocarpic, triploid and thus clonally propagated. This has resulted in a narrow genetic base and limited resilience to biotic and abiotic stresses. Mutagenesis of in vitro propagated bananas is one method to introduce novel alleles and broaden genetic diversity. We previously established a method for the induction and recovery of single nucleotide mutations generated with the chemical mutagen EMS. However, officially released mutant banana varieties have been created using gamma rays, a mutagen that can produce large genomic insertions and deletions (indels). Such dosage mutations may be important for generating observable phenotypes in polyploids. In this study, we establish a low-coverage whole-genome sequencing approach in triploid bananas to recover large genomic indels caused by treatment with gamma irradiation. We first evaluated the commercially released mutant cultivar 'Novaria' and found that it harbours multiple predicted deletions, ranging from 0.3 to 3.8 million base pairs (Mbp). In total, predicted deletions span 189 coding regions. To evaluate the feasibility of generating and maintaining new mutations, we developed a pipeline for mutagenesis and screening for copy number variation in Cavendish bananas using the cultivar 'Williams'. Putative mutations were recovered in 70% of lines treated with 20 Gy and 60% of the lines treated with 40 Gy. While deletion events predominate, insertions were identified in 20 Gy-treated material. Based on these results, we believe this approach can be scaled up to support large breeding projects.
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