ArticleScientific reports2018
An integrated microfluidic device for the sorting of yeast cells using image processing.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Microfluidics-based cell recognition through optimizing suspended cell staining techniques and artificial intelligence.Journal of biological engineering · 2026Article
- Advances in machine learning-enhanced microfluidic cell sorting.Science advances · 2025Review
- Optical sorting: past, present and future.Light, science & applications · 2025Review
- Droplet-based microfluidic platform for detecting agonistic peptides that are self-secreted by yeast expressing a G-protein-coupled receptor.Microbial cell factories · 2024Article
- Recent advances in droplet sequential monitoring methods for droplet sorting.Biomicrofluidics · 2023Review
- Microfluidic Blood Separation: Key Technologies and Critical Figures of Merit.Micromachines · 2023Review
- Microfluidic pumps for cell sorting.Biomicrofluidics · 2023Review
- Machine learning implementation strategy in imaging and impedance flow cytometry.Biomicrofluidics · 2023Review
- The role of NAD and NAD precursors on longevity and lifespan modulation in the budding yeast, Saccharomyces cerevisiae.Biogerontology · 2022Review
- Two-photon fluorescence lifetime for label-free microfluidic droplet sorting.Analytical and bioanalytical chemistry · 2022Article
- Single-Cell Technologies to Study Phenotypic Heterogeneity and Bacterial Persisters.Microorganisms · 2021Review
- Image-Based Live Cell Sorting.Trends in biotechnology · 2021Review
- On-Chip Multiple Particle Velocity and Size Measurement Using Single-Shot Two-Wavelength Differential Image Analysis.Micromachines · 2020Article
- A neural network approach for real-time particle/cell characterization in microfluidic impedance cytometry.Analytical and bioanalytical chemistry · 2020Article
- Image-Based Single Cell Sorting Automation in Droplet Microfluidics.Scientific reports · 2020Article
- Cell Separations and Sorting.Analytical chemistry · 2020Review
- Enhanced inertial focusing of microparticles and cells by integrating trapezoidal microchambers in spiral microfluidic channels.RSC advances · 2019Article
- Size Distribution Analysis with On-Chip Multi-Imaging Cell Sorter for Unlabeled Identification of Circulating Tumor Cells in Blood.Micromachines · 2019Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The process of detection and separation of yeast cells based on their morphological characteristics is critical to the understanding of cell division cycles, which is of vital importance to the understanding of some diseases such as cancer. The traditional process of manual detection is usually tedious and inconsistent. This paper presents a microfluidic device integrated with microvalves for fluid control for the sorting of yeast cells using image processing algorithms and confirmation based on their fluorescent tag. The proposed device is completely automated, low cost and easy to implement in an academic research setting. Design details of the integrated microfluidic system are highlighted in this paper, along with experimental validation. Real time cell sorting was demonstrated with a cell detection rate of 12 cells per minute.
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