ArticleStem cell research2018
Advanced immunostaining approaches to study early male germ cell development.
Article in Stem cell research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Identification and characterization of a novel CD2-positive cell population in the seminiferous tubule of Fischer CDF344 rats†.Biology of reproduction · 2025Article
- Bioactive metabolites from Paeonia lactiflora protect against heat-induced male infertility in Drosophila melanogaster by modulating Vasa: integrating in vivo and computational analyses.Journal of computer-aided molecular design · 2025Article
- Utilization of the QuPath open-source software platform for analysis of mammalian spermatogenesis†.Biology of reproduction · 2025Article
- Autophagy Analysis: A Step-by-Step Simple Practical Guide for Immunofluorescence and Western Blotting.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Cadherin-18 loss in prospermatogonia and spermatogonial stem cells enhances cell adhesion through a compensatory mechanism.Zoological research · 2024Article
- In vivo CRISPR screening directly targeting testicular cells.Cell genomics · 2024Article
- Retinoic acid is dispensable for meiotic initiation but required for spermiogenesis in the mammalian testis.Development (Cambridge, England) · 2023Article
- Pharmacological Treatment of Neonatal and Juvenile Mice to Study Spermatogenesis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- VASA protein and gene expression analysis of human non-obstructive azoospermia and normal by immunohistochemistry, immunocytochemistry, and bioinformatics analysis.Scientific reports · 2022Article
- The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice.PLoS genetics · 2022Article
- Sertoli cell and spermatogonial development in pigs.Journal of animal science and biotechnology · 2022Article
- Immunofluorescence Staining of Paraffin Sections Step by Step.Frontiers in neuroanatomy · 2020Article
- Establishment of cell lines with porcine spermatogonial stem cell properties.Journal of animal science and biotechnology · 2020Article
- Acyl-CoA synthetase 6 enriches seminiferous tubules with the ω-3 fatty acid docosahexaenoic acid and is required for male fertility in the mouse.The Journal of biological chemistry · 2019Article
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2 authors.
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Abstract
Mammalian male germ cell development takes place in the testis under the influence of a variety of somatic cells and an incompletely defined paracrine and endocrine influences. Since it is not recapitulated well in vitro, researchers studying spermatogenesis often manipulate the germline by creating transgenic or knockout mice or by administering pharmaceutical agonists/antagonists or inhibitors. The effects of these types of manipulations on germline development can often be determined following microscopic imaging, both of stained and immunostained testis sections. Here, we describe approaches for microscopic analysis of the developing male germline, provide detailed protocols for a variety of immunostaining approaches, and discuss transgenic fluorescent reporter lines for studying the early stages of spermatogenesis.
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