Scope and limitations of carbohydrate hydrolysis for de novo glycan sequencing using a hydrogen peroxide/metallopeptide-based glycosidase mimetic.

Tianyuan Peng, Zachary Wooke, Nicola L B Pohl

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In one paragraph

Article in Carbohydrate research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.2field-weighted citation impact, top 53% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

Characterization and Analysis of Food-Sourced Carbohydrates.Methods in molecular biology (Clifton, N.J.) · 2022
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Tianyuan PengDepartment of Chemistry, Indiana University, Bloomington, IN, USA.

Zachary WookeDepartment of Chemistry, Indiana University, Bloomington, IN, USA.

Nicola L B PohlDepartment of Chemistry, Indiana University, Bloomington, IN, USA; Radcliffe Institute of Advanced Study, Harvard University, Cambridge, MA, USA. Electronic address: npohl@indiana.edu.

Indiana University Bloomington · US

Funding

Sugar Building Blocks and Automated Synthesis of Biomedically-Relevant GlycansU01GM116248 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI POHL, NICOLA L. B. · 2015 to 2019

$2.7M

NIGMS NIH HHS U01 GM116248

6 · The paper itself

Abstract

Acidic hydrolysis is commonly used as a first step to break down oligo- and polysaccharides into monosaccharide units for structural analysis. While easy to set up and amenable to mass spectrometry detection, acid hydrolysis is not without its drawbacks. For example, ring-destruction side reactions and degradation products, along with difficulties in optimizing conditions from analyte to analyte, greatly limits its broad utility. Herein we report studies on a hydrogen peroxide/CuGGH metallopeptide-based glycosidase mimetic design for a more efficient and controllable carbohydrate hydrolysis. A library of methyl glycosides consisting of ten common monosaccharide substrates, along with oligosaccharide substrates, was screened with the artificial glycosidase for hydrolytic activity in a high-throughput format with a robotic liquid handling system. The artificial glycosidase was found to be active towards most screened linkages, including alpha- and beta-anomers, thus serving as a potential alternative method for traditional acidic hydrolysis approaches of oligosaccharides.

Indexed as

CarbohydratesGlycoside HydrolasesHydrogen PeroxideHydrolysisPolysaccharidesCarbohydratesGlycoside HydrolasesHydrogen PeroxidePolysaccharidesArtificial glycosidaseCarbohydrate hydrolysisCarbohydrate sequencingHigh-throughput screening

Identifiers

PMID29475194

DOI10.1016/j.carres.2018.01.008

PMCPMC5931371

OpenAlexW2789862187

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.