ArticleJournal of experimental botany2018
The transcription factor CsbHLH18 of sweet orange functions in modulation of cold tolerance and homeostasis of reactive oxygen species by regulating the antioxidant gene.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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54 citing papers in PubMed, 155 citations in OpenAlex.
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- An R2R3-MYB transcription factor TgMYB44 positively regulates petal senescence by enhancing ABA biosynthesis in tulip.Plant cell reports · 2026Article
- Identification of Chalcone Synthase Genes and Their Responses to Salt and Cold Stress inPlants (Basel, Switzerland) · 2025Article
- From Hormones to Harvests: A Pathway to Strengthening Plant Resilience for Achieving Sustainable Development Goals.Plants (Basel, Switzerland) · 2025Review
- Natural allelic variation of basic helix-loop-helix transcription factor 25 regulates carotenoid biosynthesis in sweet potato.Plant biotechnology journal · 2025Article
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- Molecular mechanism of response to low-temperature during the natural overwintering period of Rosa persica.Plant cell reports · 2025Article
- A bHLH transcription factor RrUNE12 regulates salt tolerance and promotes ascorbate synthesis.Plant cell reports · 2025Article
- Genome-wide identification, phylogenetic and expression pattern analysis of GATA gene family inFrontiers in plant science · 2025Article
- Metabolome and transcriptome analyses reveal molecular responses of two potato (Frontiers in plant science · 2025Article
- Genome-wide characterization and expression profiling of theFrontiers in plant science · 2025Article
- The GRAS transcription factor PtrPAT1 ofHorticulture research · 2025Article
- Functions and Regulatory Mechanisms of bHLH Transcription Factors during the Responses to Biotic and Abiotic Stresses in Woody Plants.Plants (Basel, Switzerland) · 2024Review
- Genome-wide identification of the GRF family in sweet orange (Citrus sinensis) and functional analysis of the CsGRF04 in response to multiple abiotic stresses.BMC genomics · 2024Article
- Full-length single-molecule sequencing uncovers novel insight into the global landscape of the cold stress response in trifoliate orange (Frontiers in plant science · 2024Article
- Functional analysis of sweet cherry PavbHLH106 in the regulation of cold stress.Plant cell reports · 2023Article
- Overexpression of PavbHLH28 from Prunus avium enhances tolerance to cold stress in transgenic Arabidopsis.BMC plant biology · 2023Article
- OverexpressingInternational journal of molecular sciences · 2023Article
- Kiwifruit bZIP transcription factor AcePosF21 elicits ascorbic acid biosynthesis during cold stress.Plant physiology · 2023Article
- Transcriptional and Post-Translational Regulation of Plant bHLH Transcription Factors during the Response to Environmental Stresses.Plants (Basel, Switzerland) · 2023Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The basic helix-loop-helix (bHLH) transcription factors (TFs) comprise one of the largest gene families in plants, and participate in various physiological processes, but the physiological role and regulatory function of the majority of bHLHs remain poorly understood. Here, a total of 56 putative CsbHLH genes were identified in sweet orange (Citrus sinensis) based on a genome-wide analysis. The CsbHLH genes, except four members, were distributed throughout nine chromosomes and divided into 19 subgroups. Most of the CsbHLH genes were responsive to cold stress, with the greatest up-regulation being observed in CsbHLH18. CsbHLH18 is localized in the nuclei and has transcriptional activation activity. Overexpression of CsbHLH18 conferred enhanced cold tolerance in transgenic tobacco. The transgenic plants accumulated significantly less reactive oxygen species (ROS), concurrent with increased activities and transcript levels of antioxidant enzymes. In contrast, knockdown of bHLH18 by RNAi in trifoliate orange promoted cold susceptibility, accompanied by down-regulation of antioxidant genes and accumulation of more ROS. Protein-DNA interaction assays demonstrate that CsbHLH18 directly and specifically binds to and activates the promoter of CsPOD. Taken together, these findings indicate that CsbHLH18 plays a positive role in cold tolerance through, at least partly, modulation of ROS homeostasis by directly regulating the antioxidant gene.
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