ArticlePloS one2018
Transcriptome analysis in different developmental stages of Batocera horsfieldi (Coleoptera: Cerambycidae) and comparison of candidate olfactory genes.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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21 citing papers in PubMed.
- De novo transcriptome meta-analysis reveals candidate genes involved in life-stage transitions for RNAi-mediated management of the citrus root weevil (Diaprepes abbreviatus).Pest management science · 2026Article
- Genome-Wide Identification and Characterization of Chemosensory Gene Families in the MayflyGenes · 2026Article
- Transcriptome analysis used to identify and characterize odorant binding proteins in Agasicles hygrophila (Coleoptera: Chryspmelidae).Journal of insect science (Online) · 2023Article
- SMRT Sequencing Technology Was Used to Construct theInsects · 2023Article
- The complete mitochondrial genome ofMitochondrial DNA. Part B, Resources · 2023Article
- Antennal transcriptome analysis of olfactory genes and tissue expression profiling of odorant binding proteins in Semanotus bifasciatus (cerambycidae: coleoptera).BMC genomics · 2022Article
- Chromosome-level genome assembly, annotation, and phylogenomics of the gooseneck barnacle Pollicipes pollicipes.GigaScience · 2022Article
- Transcriptome analysis ofFrontiers in physiology · 2022Article
- Phylogenetic Analysis of Small Hive Beetles From Native to Introduced Populations.Frontiers in genetics · 2022Article
- Comparative analyses of transcriptional responses of Dectes texanus LeConte (Coleoptera: Cerambycidae) larvae fed on three different host plants and artificial diet.Scientific reports · 2021Article
- Comparative Transcriptomic Analysis ofInsects · 2021Article
- The Developmental Transcriptome of Bagworm,Genes · 2020Article
- Transcriptome Sequencing of the Striped Cucumber Beetle,Biotech (Basel (Switzerland)) · 2020Article
- Deciphering the molecular mechanisms of mother-to-egg immune protection in the mealworm beetle Tenebrio molitor.PLoS pathogens · 2020Article
- Dataset ofData in brief · 2020Article
- Transcriptome and gene expression analysis ofPeerJ · 2020Article
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- The whole body transcriptome ofPeerJ · 2020Article
- Comparisons of lung and gluteus transcriptome profiles between yaks at different ages.Scientific reports · 2019Article
- Transcriptome and gene expression analysis of three developmental stages of the coffee berry borer, Hypothenemus hampei.Scientific reports · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The white-striped longhorn beetle Batocera horsfieldi (Coleoptera: Cerambycidae) is a polyphagous wood-boring pest that causes substantial damage to the lumber industry. Moreover olfactory proteins are crucial components to function in related processes, but the B. horsfieldi genome is not readily available for olfactory proteins analysis. In the present study, developmental transcriptomes of larvae from the first instar to the prepupal stage, pupae, and adults (females and males) from emergence to mating were built by RNA sequencing to establish a genetic background that may help understand olfactory genes. Approximately 199 million clean reads were obtained and assembled into 171,664 transcripts, which were classified into 23,380, 26,511, 22,393, 30,270, and 87, 732 unigenes for larvae, pupae, females, males, and combined datasets, respectively. The unigenes were annotated against NCBI's non-redundant nucleotide and protein sequences, Swiss-Prot, Gene Ontology (GO), Pfam, Clusters of Eukaryotic Orthologous Groups (KOG), and KEGG Orthology (KO) databases. A total of 43,197 unigenes were annotated into 55 sub-categories under the three main GO categories; 25,237 unigenes were classified into 26 functional KOG categories, and 25,814 unigenes were classified into five functional KEGG Pathway categories. RSEM software identified 2,983, 3,097, 870, 2,437, 5,161, and 2,882 genes that were differentially expressed between larvae and males, larvae and pupae, larvae and females, males and females, males and pupae, and females and pupae, respectively. Among them, genes encoding seven candidate odorant binding proteins (OBPs) and three chemosensory proteins (CSPs) were identified. RT-PCR and RT-qPCR analyses showed that BhorOBP3, BhorCSP2, and BhorOBPC1/C3/C4 were highly expressed in the antenna of males, indicating these genes may may play key roles in foraging and host-orientation in B. horsfieldi. Our results provide valuable molecular information about the olfactory system in B. horsfieldi and will help guide future functional studies on olfactory genes.
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