ArticleScientific reports2018
Endogenous insensitivity to the Orco agonist VUAA1 reveals novel olfactory receptor complex properties in the specialist fly Mayetiola destructor.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 45 citations in OpenAlex.
- Cryo-EM structures of Drosophila OR67d-Orco complexes reveal insect pheromone sensing mechanism.Cell research · 2026Article
- Molecular bases of insect odorant receptor function: specificity and evolution.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Highly Expressed Odorant Receptor Orthologs Detect the Aggregation Pheromone Lineatin in Trypodendron Ambrosia Beetles.Molecular ecology · 2025Article
- Alternative strategies based on transgenic Drosophila melanogaster for the functional characterization of insect Ionotropic Receptors.Biological research · 2025Article
- Life-stage dependent behavior mimics chemosensory repertoire diversity in a belowground, specialist herbivore.G3 (Bethesda, Md.) · 2025Article
- Eurasian spruce bark beetle detects lanierone using a highly expressed specialist odorant receptor, present in several functional sensillum types.BMC biology · 2024Article
- Functional Characterization Supports Multiple Evolutionary Origins of Pheromone Receptors in Bark Beetles.Molecular biology and evolution · 2024Article
- Identification of a receptor for the sex pheromone of the vine mealybug,Current research in insect science · 2024Article
- Activity of Single Insect Olfactory Receptors Triggered by Airborne Compounds Recorded in Self-Assembled Tethered Lipid Bilayer Nanoarchitectures.ACS applied materials & interfaces · 2023Article
- The chemosensory system of theFly · 2022Review
- Odorant receptor orthologues in conifer-feeding beetles display conserved responses to ecologically relevant odours.Molecular ecology · 2022Article
- Functional conservation of Anopheline linalool receptors through 100 million years of evolution.Chemical senses · 2022Article
- Impacts of OrX and cAMP-insensitive Orco to the insect olfactory heteromer activity.Molecular biology reports · 2021Article
- Altered functional properties of the codling moth Orco mutagenized in the intracellular loop-3.Scientific reports · 2021Article
- Putative ligand binding sites of two functionally characterized bark beetle odorant receptors.BMC biology · 2021Article
- Codon Optimization of Insect Odorant Receptor Genes May Increase Their Stable Expression for Functional Characterization in HEK293 Cells.Frontiers in cellular neuroscience · 2021Article
- Functional properties of insect olfactory receptors: ionotropic receptors and odorant receptors.Cell and tissue research · 2021Review
- Use of machine learning to identify novel, behaviorally active antagonists of the insect odorant receptor co-receptor (Orco) subunit.Scientific reports · 2019Article
- Article
- Antennal Transcriptome Analysis of the Chemosensory Gene Families From Trichoptera and Basal Lepidoptera.Frontiers in physiology · 2018Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insect olfactory receptors are routinely expressed in heterologous systems for functional characterisation. It was recently discovered that the essential olfactory receptor co-receptor (Orco) of the Hessian fly, Mayetiola destructor (Mdes), does not respond to the agonist VUAA1, which activates Orco in all other insects analysed to date. Here, using a mutagenesis-based approach we identified three residues in MdesOrco, located in different transmembrane helices as supported by 3D modelling, that confer sensitivity to VUAA1. Reciprocal mutations in Drosophila melanogaster (Dmel) and the noctuid moth Agrotis segetum (Aseg) Orcos diminish sensitivity of these proteins to VUAA1. Additionally, mutating these residues in DmelOrco and AsegOrco compromised odourant receptor (OR) dependent ligand-induced Orco activation. In contrast, both wild-type and VUAA1-sensitive MdesOrco were capable of forming functional receptor complexes when coupled to ORs from all three species, suggesting unique complex properties in M. destructor, and that not all olfactory receptor complexes are "created" equal.
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