ArticleNature communications2018
Programmed hierarchical patterning of bacterial populations.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Assembly of functional microbial ecosystems: from molecular circuits to communities.FEMS microbiology reviews · 2024Review
- Construction of the genetic switches in response to mannitol based on artificial MtlR box.Bioresources and bioprocessing · 2023Article
- Advances and challenges in programming pattern formation using living cells.Current opinion in chemical biology · 2022Review
- Synthetic spatial patterning in bacteria: advances based on novel diffusible signals.Microbial biotechnology · 2022Review
- Bespoke Turing Systems.Bulletin of mathematical biology · 2021Article
- Interpretation of morphogen gradients by a synthetic bistable circuit.Nature communications · 2020Article
- Turing Patterning in Stratified Domains.Bulletin of mathematical biology · 2020Article
- Engineering pattern formation and morphogenesis.Biochemical Society transactions · 2020Review
- Controlling spatiotemporal pattern formation in a concentration gradient with a synthetic toggle switch.Molecular systems biology · 2020Article
- Engineered cell-to-cell signalling within growing bacterial cellulose pellicles.Microbial biotechnology · 2019Article
- Recent Advances and Current Challenges in Synthetic Biology of the Plastid Genetic System and Metabolism.Plant physiology · 2019Review
- Synthetic Biology Tools to Engineer Microbial Communities for Biotechnology.Trends in biotechnology · 2019Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Modern genetic tools allow the dissection and emulation of fundamental mechanisms shaping morphogenesis in multicellular organisms. Several synthetic genetic circuits for control of multicellular patterning have been reported to date. However, hierarchical induction of gene expression domains has received little attention from synthetic biologists, despite its importance in biological self-organization. Here we report a synthetic genetic system implementing population-based AND-logic for programmed autonomous induction of bacterial gene expression domains. We develop a ratiometric assay for bacteriophage T7 RNA polymerase activity and use it to systematically characterize different intact and split enzyme variants. We then utilize the best-performing variant to build a three-color patterning system responsive to two different homoserine lactones. We validate the AND gate-like behavior of this system both in cell suspension and in surface culture. Finally, we use the synthetic circuit in a membrane-based spatial assay to demonstrate programmed hierarchical patterning of gene expression across bacterial populations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.