ArticleParasites & vectors2018
Multilocus sequence typing: genetic diversity in Trypanosoma cruzi I (TcI) isolates from Brazilian didelphids.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
- Current and emerging molecular diagnostic approaches in the detection of human parasites.Parasitology research · 2026Review
- The Relevance of the Predominant Clonal Evolution (PCE) Model for the Molecular Epidemiology and Subspecific Taxonomy ofPathogens (Basel, Switzerland) · 2025Review
- "Mi Casa, Tu Casa": the coati nest as a hub of Trypanosoma cruzi transmission in the southern Pantanal biome revealed by molecular blood meal source identification in triatomines.Parasites & vectors · 2023Article
- Old Methods, New Insights: Reviewing Concepts on the Ecology of Trypanosomatids andPathogens (Basel, Switzerland) · 2023Article
- Comparison of four multilocus sequence typing schemes and amino acid biosynthesis based on genomic analysis of Bacillus subtilis.PloS one · 2023Article
- Discrimination ofJournal of microbiology and biotechnology · 2022Article
- Comparison of ITS-1 and TBR-1/2 primer sensitivity for the detection ofVeterinary world · 2022Article
- Trypanosomatid Richness Among Rats, Opossums, and Dogs in the Caatinga Biome, Northeast Brazil, a Former Endemic Area of Chagas Disease.Frontiers in cellular and infection microbiology · 2022Article
- Trypanosomatid Richness in Wild and Synanthropic Small Mammals from a Biological Station in Rio de Janeiro, Brazil.Pathogens (Basel, Switzerland) · 2021Article
- Trypanosomatid species in Didelphis albiventris from urban forest fragments.Parasitology research · 2021Article
- Prevalence of Trypanosoma evansi in livestock in Palestine.Parasites & vectors · 2020Article
- Landmarks of the Knowledge andFrontiers in cellular and infection microbiology · 2020Review
- Multilocus Sequence Typing (MLST) and Random Polymorphic DNA (RAPD) Comparisons of Geographic Isolates ofPathogens (Basel, Switzerland) · 2019Article
- Trypanosomatids in Small Mammals of an Agroecosystem in Central Brazil: Another Piece in the Puzzle of Parasite Transmission in an Anthropogenic Landscape.Pathogens (Basel, Switzerland) · 2019Article
- The influence of parasitism byInternational journal for parasitology. Parasites and wildlife · 2019Article
- UncoveringInternational journal for parasitology. Parasites and wildlife · 2019Article
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4 authors.
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Abstract
backgroundTrypanosoma cruzi is a protozoan parasite characterized by extensive genetic heterogeneity. There are currently six recognised, genetically distinct, monophyletic clades designated discrete typing units (DTUs). TcI has the broadest geographical range and most genetic diversity evidenced by a wide range of genetic markers applied to isolates spanning a vast geographical range across Latin America. However, little is known of the diversity of TcI that exists within sylvatic mammals across the geographical expanse of Brazil.
resultsTwenty-nine sylvatic TcI isolates spanning multiple ecologically diverse biomes across Brazil were analyzed by the application of multilocus sequence typing (MLST) using four nuclear housekeeping genes. Results revealed extensive genetic diversity and also incongruence among individual gene trees. There was no association of intralineage genotype with geography or with any particular biome, with the exception of isolates from Caatinga that formed a single cluster. However, haplotypic analyses of METIII and LYT1 constitutive markers provided evidence of recombination events in two isolates derived from Didelphis marsupialis and D. albiventris, respectively. For diversity studies all possible combinations of markers were assessed with the objective of selecting the combination of gene targets that are most resolutive using the minimum number of genes. A panel of just three gene fragments (DHFR-TS, LYT1 and METIII) discriminated 26 out of 35 genotypes.
conclusionsThese findings showed geographical association of genotypes clustering in Caatinga but more characteristically TcI genotypes widely distributed without specific association to geographical areas or biomes. Importantly, we detected the signature of recombination events at the nuclear level evidenced by haplotypic analysis and incongruence.
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