ArticleBMC genomics2018
Gene expression correlated with delay in shell formation in larval Pacific oysters (Crassostrea gigas) exposed to experimental ocean acidification provides insights into shell formation mechanisms.
Article in BMC genomics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 72 citations in OpenAlex.
- Comparing Minimally Lethal Sampling Methods for Genomics in the Eastern Oyster (Ecology and evolution · 2026Article
- Primordial germ cell specification and early developmental cell states in Pacific oyster.BMC genomics · 2025Article
- Transcriptome-To-Phenome Response of Larval Eastern Oysters Under Multiple Drivers of Aragonite Undersaturation.Ecology and evolution · 2025Article
- Core genes of biomineralization and cis-regulatory long non-coding RNA regulate shell growth in bivalves.Journal of advanced research · 2024Article
- Comparative transcriptome analysis reveals molecular damage associated with cryopreservation in Crassostrea angulata D-larvae rather than to cryoprotectant exposure.BMC genomics · 2024Article
- Consequences of domestication in eastern oyster: Insights from whole genomic analyses.Evolutionary applications · 2024Article
- Article
- Combination of RNAseq and RADseq to Identify Physiological and Adaptive Responses to Acidification in the Eastern Oyster (Crassostrea virginica).Marine biotechnology (New York, N.Y.) · 2023Article
- RNAi Silencing of the Biomineralization Gene Perlucin Impairs Oyster Ability to Cope with Ocean Acidification.International journal of molecular sciences · 2023Article
- Directional fabrication and dissolution of larval and juvenile oyster shells under ocean acidification.Proceedings. Biological sciences · 2023Article
- Proteomic and Transcriptomic Responses Enable Clams to Correct the pH of Calcifying Fluids and Sustain Biomineralization in Acidified Environments.International journal of molecular sciences · 2022Article
- Convergent evolution of barnacles and molluscs sheds lights in origin and diversification of calcareous shell and sessile lifestyle.Proceedings. Biological sciences · 2022Article
- Identification and Characterization of the Larval Settlement Pheromone Protein Components in Adult Shells ofInternational journal of molecular sciences · 2022Article
- Article
- Differential DNA methylation in Pacific oyster reproductive tissue in response to ocean acidification.BMC genomics · 2022Article
- Ocean Acidification Alters Developmental Timing and Gene Expression of Ion Transport Proteins During Larval Development in Resilient and Susceptible Lineages of the Pacific Oyster (Crassostrea gigas).Marine biotechnology (New York, N.Y.) · 2022Article
- Draft genome of Semisulcospira libertina, a species of freshwater snail.Genomics & informatics · 2021Article
- Larval development in the Pacific oyster and the impacts of ocean acidification: Differential genetic effects in wild and domesticated stocks.Evolutionary applications · 2021Article
- Transcriptomic analysis of shell repair and biomineralization in the blue mussel, Mytilus edulis.BMC genomics · 2021Article
- Single-Larva RNA Sequencing Identifies Markers of Copper Toxicity and Exposure in EarlyFrontiers in physiology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundDespite recent work to characterize gene expression changes associated with larval development in oysters, the mechanism by which the larval shell is first formed is still largely unknown. In Crassostrea gigas, this shell forms within the first 24 h post fertilization, and it has been demonstrated that changes in water chemistry can cause delays in shell formation, shell deformations and higher mortality rates. In this study, we use the delay in shell formation associated with exposure to CO
resultsBy fitting linear models to gene expression data in ambient and low aragonite saturation treatments, we are able to isolate 37 annotated genes correlated with initial larval shell formation, which can be categorized into 1) ion transporters, 2) shell matrix proteins and 3) protease inhibitors. Clustering of the gene expression data into co-expression networks further supports the result of the linear models, and also implies an important role of dynein motor proteins as transporters of cellular components during the initial shell formation process.
conclusionsUsing an RNA-Seq approach with high temporal resolution allows us to identify a conceptual model for how oyster larval calcification is initiated. This work provides a foundation for further studies on how genetic variation in these identified genes could affect fitness of oyster populations subjected to future environmental changes, such as ocean acidification.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.