ArticleJournal of experimental botany2018
Novel roles of ascorbate in plants: induction of cytosolic Ca2+ signals and efflux from cells via anion channels.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 95 citations in OpenAlex.
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- Multiple Physiological and Biochemical Functions of Ascorbic Acid in Plant Growth, Development, and Abiotic Stress Response.International journal of molecular sciences · 2024Review
- The Functions of Chloroplastic Ascorbate in Vascular Plants and Algae.International journal of molecular sciences · 2023Review
- Comparative Transcriptomic Analysis of Root and Leaf Transcript Profiles Reveals the Coordinated Mechanisms in Response to Salinity Stress in Common Vetch.International journal of molecular sciences · 2022Article
- Label-Free Quantitative Proteomics Unravel the Impacts of Salt Stress onFrontiers in plant science · 2022Article
- The Role of Calcium/Calcium-Dependent Protein Kinases Signal Pathway in Pollen Tube Growth.Frontiers in plant science · 2021Review
- Ecological and Geochemical Conditions for the Accumulation of Antioxidants in the Leaves ofPlants (Basel, Switzerland) · 2020Article
- Sodium Influx and Potassium Efflux Currents in Sunflower Root Cells Under High Salinity.Frontiers in plant science · 2020Article
- Regulation of Ascorbate-Glutathione Pathway in Mitigating Oxidative Damage in Plants under Abiotic Stress.Antioxidants (Basel, Switzerland) · 2019Review
- Glycation of Plant Proteins: Regulatory Roles and Interplay with Sugar Signalling?International journal of molecular sciences · 2019Review
- Biochemical mechanisms regulating salt tolerance in sunflower.Plant signaling & behavior · 2019Review
- Modulation of Ethylene and Ascorbic Acid on Reactive Oxygen Species Scavenging in Plant Salt Response.Frontiers in plant science · 2019Review
- Early Extracellular ATP Signaling inFrontiers in plant science · 2019Article
- The Dynamic Responses of Cell Walls in Resurrection Plants During Dehydration and Rehydration.Frontiers in plant science · 2019Review
- Ascorbic acid metabolism and functions: A comparison of plants and mammals.Free radical biology & medicine · 2018Review
- ROS and RNS: key signalling molecules in plants.Journal of experimental botany · 2018Article
- Powering the plasma membrane Ca2+-ROS self-amplifying loop.Journal of experimental botany · 2018Article
- ROS-Activated Ion Channels in Plants: Biophysical Characteristics, Physiological Functions and Molecular Nature.International journal of molecular sciences · 2018Review
- An Anion Conductance, the Essential Component of the Hydroxyl-Radical-Induced Ion Current in Plant Roots.International journal of molecular sciences · 2018Article
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Authors and funding
12 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ascorbate is not often considered as a signalling molecule in plants. This study demonstrates that, in Arabidopsis roots, exogenous l-ascorbic acid triggers a transient increase of the cytosolic free calcium activity ([Ca2+]cyt.) that is central to plant signalling. Exogenous copper and iron stimulate the ascorbate-induced [Ca2+]cyt. elevation, while cation channel blockers, free radical scavengers, low extracellular [Ca2+], transition metal chelators, and removal of the cell wall inhibit this reaction. These data show that apoplastic redox-active transition metals are involved in the ascorbate-induced [Ca2+]cyt. elevation. Exogenous ascorbate also induces a moderate increase in programmed cell death symptoms in intact roots, but it does not activate Ca2+ influx currents in patch-clamped root protoplasts. Intriguingly, the replacement of gluconate with ascorbate in the patch-clamp pipette reveals a large ascorbate efflux current, which shows sensitivity to the anion channel blocker, anthracene-9-carboxylic acid (A9C), indicative of the ascorbate release via anion channels. EPR spectroscopy measurements demonstrate that salinity (NaCl) triggers the accumulation of root apoplastic ascorbyl radicals in an A9C-dependent manner, confirming that l-ascorbate leaks through anion channels under depolarization. This mechanism may underlie ascorbate release, signalling phenomena, apoplastic redox reactions, iron acquisition, and control the ionic and electrical equilibrium (together with K+ efflux via GORK channels).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.