ArticleMolecular microbiology2018
Genes affecting progression of bacteriophage P22 infection in Salmonella identified by transposon and single gene deletion screens.
Article in Molecular microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Whole genome sequence-based comparative genomics reveals preliminary genomic features of Salmonella enterica subsp. enterica serovar Enteritidis phage type 1 and phage type 4 strains from EnteroBase.BMC microbiology · 2026Article
- Structure and function of Salmonella inner membrane.Archives of microbiology · 2026Review
- A comprehensive study on Salmonella enterica serovar Richmond in farmed fish Pangasianodon hypophthalmus: insights into zoonotic potential, virulence and antimicrobial resistance.Folia microbiologica · 2026Article
- Phage Frontiers: Genomic and Functional Profiling of Novel Virulent Agents Targeting FoodborneBiology · 2026Article
- Stable co-existence ofmBio · 2026Article
- A recombinant lysogenic bacteriophage inhibitsAntimicrobial agents and chemotherapy · 2026Article
- Phage resistance mutations in a marine bacterium impact biogeochemically relevant cellular processes.Nature microbiology · 2026Article
- TheJournal of virology · 2025Article
- Systematic genome-wide mapping of host determinants of bacteriophage infectivity.Cell systems · 2025Article
- Characterization of Salmonella adaptation in response to phage treatment in broiler chickens.Veterinary research · 2025Article
- Two receptor-targeting mechanisms of lambda-like siphophage Gifsy-1 of Salmonella Typhimurium.PLoS pathogens · 2025Article
- Moo19 and B2: Structures ofScience advances · 2024Article
- PlzR regulates type IV pili assembly in Pseudomonas aeruginosa via PilZ binding.Nature communications · 2024Article
- Bacterial resistance to temperate phage is influenced by the frequency of lysogenic establishment.iScience · 2024Article
- Thinking Phage Innovations Through Evolution and Ecology.PHAGE (New Rochelle, N.Y.) · 2024Review
- Article
- The phage fought the cells, and the phage won: a satellite symposium at the ASV 2023 annual meeting.Journal of virology · 2023Article
- Therapeutically useful mycobacteriophages BPs and Muddy require trehalose polyphleates.Nature microbiology · 2023Article
- Host Range Expansion ofJournal of virology · 2022Article
- Flagellotropic Bacteriophages: Opportunities and Challenges for Antimicrobial Applications.International journal of molecular sciences · 2022Review
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Authors and funding
8 authors.
Funding
Abstract
Bacteriophages rely on their hosts for replication, and many host genes critically determine either viral progeny production or host success via phage resistance. A random insertion transposon library of 240,000 mutants in Salmonella enterica serovar Typhimurium was used to monitor effects of individual bacterial gene disruptions on bacteriophage P22 lytic infection. These experiments revealed candidate host genes that alter the timing of phage P22 propagation. Using a False Discovery Rate of < 0.1, mutations in 235 host genes either blocked or delayed progression of P22 lytic infection, including many genes for which this role was previously unknown. Mutations in 77 genes reduced the survival time of host DNA after infection, including mutations in genes for enterobacterial common antigen (ECA) synthesis and osmoregulated periplasmic glucan (OPG). We also screened over 2000 Salmonella single gene deletion mutants to identify genes that impacted either plaque formation or culture growth rates. The gene encoding the periplasmic membrane protein YajC was newly found to be essential for P22 infection. Targeted mutagenesis of yajC shows that an essentially full-length protein is required for function, and potassium efflux measurements demonstrated that YajC is critical for phage DNA ejection across the cytoplasmic membrane.
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