ArticleCurrent genetics2018
Two genes encoding GH10 xylanases are essential for the virulence of the oomycete plant pathogen Phytophthora parasitica.
Article in Current genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- Genome-Wide Characterization of thePlants (Basel, Switzerland) · 2026Article
- A Fusarium sacchari Glycoside Hydrolase 12 Protein FsEG1 Is a Major Virulence Factor During Sugarcane Infection and Confers Resistance to Pokkah Boeng Disease via the HIGS Strategy.Plant biotechnology journal · 2026Article
- Dual-Role GH7 Family Proteins From Verticillium dahliae Function as Virulence Factors and Intracellular Effectors Triggering NLR-Mediated Immunity.Plant, cell & environment · 2026Article
- Application of quercetin enhances tobacco resistance to Phytophthora nicotianae.Functional & integrative genomics · 2025Article
- Genome sequencing of a novel Verticillium dahliae strain (huangweibingjun).Scientific reports · 2025Article
- Deleting an xylosidase-encoding geneFrontiers in microbiology · 2024Article
- Transcriptome analysis ofFrontiers in plant science · 2024Article
- Global characterization of GH11 family xylanases genes inFrontiers in microbiology · 2024Article
- Unveiling the Secretome of the Fungal Plant PathogenJournal of fungi (Basel, Switzerland) · 2022Article
- Identification of Gene Modules and Hub Genes Associated withJournal of fungi (Basel, Switzerland) · 2022Article
- Magnaporthe oryzae Chloroplast Targeting Endo-β-1,4-Xylanase I MoXYL1A Regulates Conidiation, Appressorium Maturation and Virulence of the Rice Blast Fungus.Rice (New York, N.Y.) · 2022Article
- A Secreted Lignin Peroxidase Required for Fungal Growth and Virulence and Related to Plant Immune Response.International journal of molecular sciences · 2022Article
- Plant-microbe interactions in the apoplast: Communication at the plant cell wall.The Plant cell · 2022Article
- A rice gene encoding glycosyl hydrolase plays contrasting roles in immunity depending on the type of pathogens.Molecular plant pathology · 2022Article
- FoEG1, a secreted glycoside hydrolase family 12 protein from Fusarium oxysporum, triggers cell death and modulates plant immunity.Molecular plant pathology · 2021Article
- Devastating intimacy: the cell biology of plant-Phytophthora interactions.The New phytologist · 2020Review
- Global Characterization of GH10 Family Xylanase Genes inInternational journal of molecular sciences · 2020Article
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Abstract
Plant cell walls are pivotal battlegrounds between microbial pathogens and their hosts. To penetrate the cell wall and thereby to facilitate infection, microbial pathogens are equipped with a wide array of cell wall-degrading enzymes to depolymerize the polysaccharides in the cell wall. However, many of these enzymes and their role in the pathogenesis of microbial pathogens are not characterized, especially those from Oomycetes. In this study, we analyzed the function of four putative endo-beta-1,4-xylanase-encoding genes (ppxyn1-ppxyn4) from Phytophthora parasitica, an oomycete plant pathogen known to cause severe disease in a wide variety of plant species. All four genes belong to the glycoside hydrolase family 10 (GH10). Recombinant proteins of ppxyn1, ppxyn2, and ppxyn4 obtained from the yeast Pichia pastoris showed degrading activities toward birch wood xylan, but they behaved differently in terms of the conditions for optimal activity, thermostability, and durability. Quantitative RT-PCR revealed upregulated expression of all four genes, especially ppxyn1 and ppxyn2, during plant infection. In contrast, ppxyn3 was highly expressed in cysts and its close homolog, ppxyn4, in germinating cysts. To uncover the role of ppxyn1 and ppxyn2 in the pathogenesis of P. parasitica, we generated silencing transformants for these two genes by double-stranded RNA-mediated gene silencing. Silencing ppxyn1 and ppxyn2 reduced the virulence of P. parasitica toward tobacco (Nicotiana benthamiana) and tomato plants. These results demonstrate the crucial role of xylanase-encoding ppxyn1 and ppxyn2 in the infection process of P. parasitica.
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