ArticleAntonie van Leeuwenhoek2018
Negative pressure wound therapy reduces the motility of Pseudomonas aeruginosa and enhances wound healing in a rabbit ear biofilm infection model.
Article in Antonie van Leeuwenhoek, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 2 syntheses or guidelines pooled it, 23 citations in OpenAlex.
- Establishment and translational evaluation of animal models for skin wound healing: a systematic review.Frontiers in physiology · 2026Pooled it
- Clinical outcomes of negative pressure wound therapy with instillation vs standard negative pressure wound therapy for wounds: A meta-analysis of randomised controlled trials.International wound journal · 2023Pooled it
- Negative Pressure Wound Therapy Modulates Wound pH and Bacterial Invasion inPlastic and reconstructive surgery. Global open · 2026Article
- The role of negative pressure wound therapy-From the perspective of drainage fluid composition.Surgery open science · 2026Article
- Bacterial Motility and Its Role in Skin and Wound Infections.International journal of molecular sciences · 2023Review
- Artificial sweeteners inhibit multidrug-resistant pathogen growth and potentiate antibiotic activity.EMBO molecular medicine · 2023Article
- Review
- Circular RNA expression profiles following negative pressure wound therapy in burn wounds with experimentalBioengineered · 2022Article
- Burns and biofilms: priority pathogens and in vivo models.NPJ biofilms and microbiomes · 2021Review
- Surfaceome and Exoproteome Dynamics in Dual-SpeciesFrontiers in microbiology · 2021Article
- Biofilm-Innate Immune Interface: Contribution to Chronic Wound Formation.Frontiers in immunology · 2021Review
- Animal Models of Type III Secretion System-Mediated Pathogenesis.Pathogens (Basel, Switzerland) · 2019Review
- Negative-Pressure Wound Therapy in aBioMed research international · 2018Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Pseudomonas aeruginosa motility, virulence factors and biofilms are known to be detrimental to wound healing. The efficacy of negative pressure wound therapy (NPWT) against P. aeruginosa has been little studied, either in vitro or in vivo. The present study evaluated the effect of negative pressure (NP) on P. aeruginosa motility in vitro, and the effect of NPWT on virulence factors and biofilms in vivo. P. aeruginosa motility was quantified under different levels of NP (atmospheric pressure, - 75, - 125, - 200 mmHg) using an in vitro model. Swimming, swarming and twitching motility were significantly inhibited by NP (- 125 and - 200 mmHg) compared with atmospheric pressure (p = 0.05). Virulence factors and biofilm components were quantified in NPWT and gauze treated groups using a rabbit ear biofilm model. Biofilm structure was studied with fluorescence microscopy and scanning electron microscopy. Additionally, viable bacterial counts and histological wound healing parameters were measured. Compared with the control, NPWT treatment resulted in a significant reduction in expression of all virulence factors assayed including exotoxin A, rhamnolipid and elastase (p = 0.01). A significant reduction of biofilm components (eDNA) (p = 0.01) was also observed in the NPWT group. The reduction of biofilm matrix was verified by fluorescence- and scanning electron-microscopy. NPWT lead to better histologic parameters (p = 0.01) and decreased bacterial counts (p = 0.05) compared with the control. NPWT treatment was demonstrated to be an effective strategy to reduce virulence factors and biofilm components, which may explain the increased wound healing observed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.