ArticleFrontiers in cell and developmental biology2018
Adherent vs. Free-Floating Neural Induction by Dual SMAD Inhibition for Neurosphere Cultures Derived from Human Induced Pluripotent Stem Cells.
Article in Frontiers in cell and developmental biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Neurons in a Dish: A Review of In Vitro Cell Models for Studying Neurogenesis.Journal of neurochemistry · 2026Review
- From mice to clinical relevance: humanizing neuroscience with human-based model systems.Frontiers in cellular neuroscience · 2026Review
- The technology, opportunities, and challenges of Synthetic Biological Intelligence.Biotechnology advances · 2023Review
- Spinal Cord Organoids to Study Motor Neuron Development and Disease.Life (Basel, Switzerland) · 2023Review
- Neural Differentiation of Induced Pluripotent Stem Cells for a Xenogeneic Material-Free 3D Neurological Disease Model Neurulation from Pluripotent Cells Using a Human Hydrogel.Current issues in molecular biology · 2023Article
- Article
- Exposure to a Pathological Condition May Be Required for the Cells to Secrete Exosomes Containing mtDNA Aberration.Journal of nucleic acids · 2022Article
- Article
- Review
- Brain organoids: A promising model to assess oxidative stress-induced central nervous system damage.Developmental neurobiology · 2021Review
- Atypical Neurogenesis in Induced Pluripotent Stem Cells From Autistic Individuals.Biological psychiatry · 2021Article
- Serotonin-specific neurons differentiated from human iPSCs form distinct subtypes with synaptic protein assembly.Journal of neural transmission (Vienna, Austria : 1996) · 2021Article
- Ventral Telencephalic Patterning Protocols for Induced Pluripotent Stem Cells.Frontiers in cell and developmental biology · 2021Article
- Multiplexed analysis of neural cytokine signaling by a novel neural cell-cell interaction microchip.Lab on a chip · 2020Article
- MCF7 Spheroid Development: New Insight about Spatio/Temporal Arrangements of TNTs, Amyloid Fibrils, Cell Connections, and Cellular Bridges.International journal of molecular sciences · 2020Article
- Visualizing the Synaptic and Cellular Ultrastructure in Neurons Differentiated from Human Induced Neural Stem Cells-An Optimized Protocol.International journal of molecular sciences · 2020Article
- Inducing human induced pluripotent stem cell differentiation through embryoid bodies: A practical and stable approach.World journal of stem cells · 2020Review
- Xeno- and transgene-free reprogramming of mesenchymal stem cells toward the cells expressing neural markers using exosome treatments.PloS one · 2020Article
- In Vitro Monolayer Culture of Dispersed Neural Stem Cells on the E-Cadherin-Based Substrate with Long-Term Stemness Maintenance.ACS omega · 2019Article
- Alzheimer's in a dish - induced pluripotent stem cell-based disease modeling.Translational neurodegeneration · 2019Review
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Keeping neural stem cells under proliferation, followed by terminal differentiation, can substantially increase the number of neurons generated. With regard to the usability of proliferating neurospheres (NSPHs) cultures, adherent induction protocols have not yet been studied in comparison to embryoid body (EB)-based protocols. To compare these proctocols, neural induction of human induced pluripotent stem cells was performed by dual SMAD inhibition under both adherent and free-floating EB culture conditions. After 10 days, we transferred cells to low-attachment culture plates and proliferated them as free-floating neurospheres. RNA was collected, transcribed to cDNA and analyzed for sonic hedgehog expression that plays an important role during proliferation process. NSPHs were analyzed by immunofluorescence imaging directly and upon continued differentiation. The EB-based approach yielded in higher numbers of cells expressing the neural stem cell marker Nestin, and showed in contrast to the adherent induction protocol increased expression levels of sonic hedgehog. Although improvements to culture consistency and reliability are desirable, the EB-based protocol appears to be superior to the adherent protocol for both, the proliferation and differentiation capacity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.