ArticleMetabolic engineering communications2016
Computational metabolic engineering strategies for growth-coupled biofuel production by
Article in Metabolic engineering communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 93 citations in OpenAlex.
- Predictive Algal Systems Biology: Integrating Omics, Genome-Scale Metabolic Models, and Machine Learning.Bioengineering (Basel, Switzerland) · 2026Review
- Engineering Cyanobacteria for High-Yield Photosynthetic Isoprene Production With Long-Term Phenotypic Stability.Plant biotechnology journal · 2026Article
- Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment.Folia microbiologica · 2025Article
- A quantitative description of light-limited cyanobacterial growth using flux balance analysis.PLoS computational biology · 2024Article
- Application of Cyanobacteria as Chassis Cells in Synthetic Biology.Microorganisms · 2024Review
- Uses of Multi-Objective Flux Analysis for Optimization of Microbial Production of Secondary Metabolites.Microorganisms · 2023Review
- Mapping competitive pathways to terpenoid biosynthesis in Synechocystis sp. PCC 6803 using an antisense RNA synthetic tool.Microbial cell factories · 2023Article
- Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria.Microorganisms · 2023Review
- A systematic overexpression approach reveals native targets to increase squalene production inFrontiers in plant science · 2023Article
- Introduction to Cyanobacteria.Advances in biochemical engineering/biotechnology · 2023Article
- In Silico Design Strategies for the Production of Target Chemical Compounds Using Iterative Single-Level Linear Programming Problems.Biomolecules · 2022Article
- pSHDY: A New Tool for Genetic Engineering of Cyanobacteria.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Combinatorial assembly platform enabling engineering of genetically stable metabolic pathways in cyanobacteria.Nucleic acids research · 2021Article
- A metabolic model of Lipomyces starkeyi for predicting lipogenesis potential from diverse low-cost substrates.Biotechnology for biofuels · 2021Article
- Bioprospecting of microbial strains for biofuel production: metabolic engineering, applications, and challenges.Biotechnology for biofuels · 2021Review
- Article
- Opportunities and Challenges for Microbial Synthesis of Fatty Acid-Derived Chemicals (FACs).Frontiers in bioengineering and biotechnology · 2021Review
- Recent advances in synthetic biology of cyanobacteria for improved chemicals production.Bioengineered · 2020Review
- Genome-scale metabolic rewiring improves titers rates and yields of the non-native product indigoidine at scale.Nature communications · 2020Article
- Adaptive laboratory evolution for growth coupled microbial production.World journal of microbiology & biotechnology · 2020Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chemical and fuel production by photosynthetic cyanobacteria is a promising technology but to date has not reached competitive rates and titers. Genome-scale metabolic modeling can reveal limitations in cyanobacteria metabolism and guide genetic engineering strategies to increase chemical production. Here, we used constraint-based modeling and optimization algorithms on a genome-scale model of Synechocystis PCC6803 to find ways to improve productivity of fermentative, fatty-acid, and terpene-derived fuels. OptGene and MOMA were used to find heuristics for knockout strategies that could increase biofuel productivity. OptKnock was used to find a set of knockouts that led to coupling between biofuel and growth. Our results show that high productivity of fermentation or reversed beta-oxidation derived alcohols such as 1-butanol requires elimination of NADH sinks, while terpenes and fatty-acid based fuels require creating imbalances in intracellular ATP and NADPH production and consumption. The FBA-predicted productivities of these fuels are at least 10-fold higher than those reported so far in the literature. We also discuss the physiological and practical feasibility of implementing these knockouts. This work gives insight into how cyanobacteria could be engineered to reach competitive biofuel productivities.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.