ArticleZoological letters2018
DNA metabarcoding of spiders, insects, and springtails for exploring potential linkage between above- and below-ground food webs.
Article in Zoological letters, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Reciprocal BLUP: A Predictability-Guided Multi-Omics Framework for Plant Phenotype Prediction.Plants (Basel, Switzerland) · 2025Article
- Development of PCR Blocking Primers Enabling DNA Metabarcoding Analysis of Dietary Composition in Hematophagous Sea Lamprey.Ecology and evolution · 2025Article
- DNA metabarcoding of spider egg sacs uncovers novel insights into host parasitoid complexes and trophic networks.Scientific reports · 2025Article
- Geosmin, a Food- and Water-Deteriorating Sesquiterpenoid and Ambivalent Semiochemical, Activates Evolutionary Conserved Receptor OR11A1.Journal of agricultural and food chemistry · 2024Article
- Towards holistic insect monitoring: species discovery, description, identification and traits for all insects.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- A review of applications and limitations of using aquatic macroinvertebrate predators for biocontrol of the African malaria mosquito, Anopheles gambiae sensu lato.Parasites & vectors · 2024Review
- Diversity of Parasitoid Wasps and Comparison of Sampling Strategies in Rice Fields Using Metabarcoding.Insects · 2024Article
- Interaction network rewiring and species' contributions to community-scale flexibility.PNAS nexus · 2024Article
- Otterly delicious: Spatiotemporal variation in the diet of a recovering population of Eurasian otters (Ecology and evolution · 2023Article
- Agricultural spider decline: long-term trends under constant management conditions.Scientific reports · 2023Article
- High throughput method of 16S rRNA gene sequencing library preparation for plant root microbial community profiling.Scientific reports · 2022Article
- Field investigation- and dietary metabarcoding-based screening of arthropods that prey on primary tea pests.Ecology and evolution · 2022Article
- A comparative analysis of spider prey spectra analyzed through the next-generation sequencing of individual and mixed DNA samples.Ecology and evolution · 2021Article
- Effects of consumer surface sterilization on diet DNA metabarcoding data of terrestrial invertebrates in natural environments and feeding trials.Ecology and evolution · 2021Article
- Deciphering the diet of a wandering spider (Ecology and evolution · 2021Article
- Tiny wasps, huge diversity - A review of German Pteromalidae with new generic and species records (Hymenoptera: Chalcidoidea).Biodiversity data journal · 2021Article
- Multi-omics analysis on an agroecosystem reveals the significant role of organic nitrogen to increase agricultural crop yield.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- High-throughput sequencing for community analysis: the promise of DNA barcoding to uncover diversity, relatedness, abundances and interactions in spider communities.Development genes and evolution · 2020Review
- New mitochondrial primers for metabarcoding of insects, designed and evaluated using in silico methods.Molecular ecology resources · 2019Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundUnderstanding feedback between above- and below-ground processes of biological communities is a key to the effective management of natural and agricultural ecosystems. However, as above- and below-ground food webs are often studied separately, our knowledge of material flow and community dynamics in terrestrial ecosystems remains limited.
resultsWe developed a high-throughput sequencing method for examining how spiders link above- and below-ground food webs as generalist predators. To overcome problems related to DNA-barcoding-based analyses of arthropod-arthropod interactions, we designed spider-specific blocking primers and Hexapoda-specific primers for the selective PCR amplification of Hexapoda prey sequences from spider samples. By applying the new DNA metabarcoding framework to spider samples collected in a temperate secondary forest in Japan, we explored the structure of a food web involving 15 spider species and various taxonomic groups of Hexapoda prey. These results support the hypothesis that multiple spider species in a community can prey on both above- and below-ground prey species, potentially coupling above- and below-ground food-web dynamics.
conclusionsThe PCR primers and metabarcoding pipeline described in this study are expected to accelerate nuclear marker-based analyses of food webs, illuminating poorly understood trophic interactions in ecosystems.
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