ArticleScientific reports2018
Direct DNA and RNA detection from large volumes of whole human blood.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Development of Universal Primer Sets for Zika Virus Envelope Gene Amplification and Sequencing.International journal of molecular sciences · 2026Article
- Amplification of RNA for identification of Zika and HCV in whole blood.Science advances · 2026Article
- Evaluating the influence of different anticoagulant blood collection tubes on the efficiency of circulating RNA extraction and Real-Time PCR quantification.Molecular biology reports · 2026Article
- A rapid detection system for core virulence and resistance genes in hypervirulentFrontiers in microbiology · 2026Article
- Rapid and Cost-EffectiveDiagnostics (Basel, Switzerland) · 2025Article
- Point-of-care detection of Burkholderia pseudomallei: A method integrating LAMP and LFSA with RNase HII hydrolysis.PLoS neglected tropical diseases · 2025Article
- Ultrasensitive detection of intact SARS-CoV-2 particles in complex biofluids using microfluidic affinity capture.Science advances · 2025Article
- Circulating tumor DNA fraction predicts residual cancer burden post-neoadjuvant chemotherapy in triple negative breast cancer.The journal of liquid biopsy · 2024Article
- Capture and Protection of Environmental DNA in a Metal-Organic Framework.Small science · 2024Article
- Dried Blood Matrix as a New Material for the Detection of DNA Viruses.Advanced healthcare materials · 2024Article
- Preanalytical Impact of Incomplete KDiagnostics (Basel, Switzerland) · 2024Article
- Recommendations for detection, validation, and evaluation of RNA editing events in cardiovascular and neurological/neurodegenerative diseases.Molecular therapy. Nucleic acids · 2024Review
- Recent advances in RNA sample preparation techniques for the detection of SARS-CoV-2 in saliva and gargle.Trends in analytical chemistry : TRAC · 2023Review
- Direct-from-Blood Detection of Pathogens: a Review of Technology and Challenges.Journal of clinical microbiology · 2023Review
- Article
- Promise and perils of paper-based point-of-care nucleic acid detection for endemic and pandemic pathogens.Lab on a chip · 2023Review
- A standardized method for plasma extracellular vesicle isolation and size distribution analysis.PloS one · 2023Article
- Filtration-assisted magnetofluidic cartridge platform for HIV RNA detection from blood.Lab on a chip · 2022Article
- Article
- Pre-analytical Practices in the Molecular Diagnostic Tests, A Concise Review.Iranian journal of pathology · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
PCR inhibitors in clinical specimens negatively affect the sensitivity of diagnostic PCR and RT-PCR or may even cause false-negative results. To overcome PCR inhibition, increase the sensitivity of the assays and simplify the detection protocols, simple methods based on quantitative nested real-time PCR and RT-PCR were developed to detect exogenous DNA and RNA directly from large volumes of whole human blood (WHB). Thermus thermophilus (Tth) polymerase is resistant to several common PCR inhibitors and exhibits reverse transcriptase activity in the presence of manganese ions. In combination with optimized concentrations of magnesium ions and manganese ions, Tth polymerase enabled efficient detection of DNA and RNA from large volumes of WHB treated with various anticoagulants. The applicability of these methods was further demonstrated by examining WHB specimens collected from different healthy individuals and those stored under a variety of conditions. The detection limit of these methods was determined by detecting exogenous DNA, RNA, and bacteria spiked in WHB. To the best of our knowledge, direct RNA detection from large volumes of WHB has not been reported. The results of the developed methods can be obtained within 4 hours, making them possible for rapid and accurate detection of disease-causing agents from WHB.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.