ArticleACS chemical biology2018
Chemiluminescent Biosensors for Detection of Second Messenger Cyclic di-GMP.
Article in ACS chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 56 citations in OpenAlex.
- Toolbox of FRET-based c-di-GMP biosensors and its FRET-To-Sort application for genome-wide mapping of c-di-GMP regulation.Nature communications · 2026Article
- Electrochemical and Optical Carbon Dots and Glassy Carbon Biosensors: A Review on Their Development and Applications in Early Cancer Detection.Micromachines · 2025Review
- Development of Malate Biosensor-Containing Hydrogels and Living Cell-Based Sensors.International journal of molecular sciences · 2024Article
- A Label-Free Approach for Relative Spatial Quantitation of c-di-GMP in Microbial Biofilms.Analytical chemistry · 2024Article
- A genetically encoded fluorescent biosensor for sensitive detection of cellular c-di-GMP levels inFrontiers in chemistry · 2024Article
- Fatty Acid Synthase Inhibitors Enhance Microtubule-Stabilizing and Microtubule-Destabilizing Drugs in Taxane-Resistant Prostate Cancer Cells.ACS pharmacology & translational science · 2023Article
- A label-free approach for relative spatial quantitation of c-di-GMP in microbial biofilms.bioRxiv : the preprint server for biology · 2023Article
- Selection of allosteric dnazymes that can sense phenylalanine by expression-SELEX.Nucleic acids research · 2023Article
- Visualization of c-di-GMP in multicellularFrontiers in cell and developmental biology · 2023Article
- Integration of an Expression Platform in the SELEX Cycle to Select DNA Aptamer Binding to a Disease Biomarker.ACS omega · 2022Article
- Simpler Procedure and Improved Performance for Pathogenic Bacteria Analysis with a Paper-Based Ratiometric Fluorescent Sensor Array.Analytical chemistry · 2022Article
- Analyzing signaling activity and function in hematopoietic cells.The Journal of experimental medicine · 2021Review
- Guanidine Biosensors Enable Comparison of Cellular Turn-on Kinetics of Riboswitch-Based Biosensor and Reporter.ACS synthetic biology · 2021Article
- A Review on Biosensors and Recent Development of Nanostructured Materials-Enabled Biosensors.Sensors (Basel, Switzerland) · 2021Review
- Riboswitch-Mediated Detection of Metabolite Fluctuations During Live Cell Imaging of Bacteria.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Tracking the homeostasis of second messenger cyclic-di-GMP in bacteria.Biophysical reviews · 2020Review
- Development of Ratiometric Bioluminescent Sensors forACS chemical biology · 2020Article
- Chemiluminescent sensors for quantitation of the bacterial second messenger cyclic di-GMP.Methods in enzymology · 2020Article
- Ratiometric BRET Measurements of ATP with a Genetically-Encoded Luminescent Sensor.Sensors (Basel, Switzerland) · 2019Article
- Fluorescent Detection of the Ubiquitous Bacterial Messenger 3',5' Cyclic Diguanylic Acid by Using a Small Aromatic Molecule.Frontiers in microbiology · 2019Article
Corrections and comments
- Commented on by
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Bacteria colonize highly diverse and complex environments, from gastrointestinal tracts to soil and plant surfaces. This colonization process is controlled in part by the intracellular signal cyclic di-GMP, which regulates bacterial motility and biofilm formation. To interrogate cyclic di-GMP signaling networks, a variety of fluorescent biosensors for live cell imaging of cyclic di-GMP have been developed. However, the need for external illumination precludes the use of these tools for imaging bacteria in their natural environments, including in deep tissues of whole organisms and in samples that are highly autofluorescent or photosensitive. The need for genetic encoding also complicates the analysis of clinical isolates and environmental samples. Toward expanding the study of bacterial signaling to these systems, we have developed the first chemiluminescent biosensors for cyclic di-GMP. The biosensor design combines the complementation of split luciferase (CSL) and bioluminescence resonance energy transfer (BRET) approaches. Furthermore, we developed a lysate-based assay for biosensor activity that enabled reliable high-throughput screening of a phylogenetic library of 92 biosensor variants. The screen identified biosensors with very large signal changes (∼40- and 90-fold) as well as biosensors with high affinities for cyclic di-GMP ( K
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.