ArticlePloS one2018
Analysis of aluminum toxicity in Hordeum vulgare roots with an emphasis on DNA integrity and cell cycle.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 58 citations in OpenAlex.
- Exclusion versus detoxification: contrasting molecular strategies of aluminium tolerance in rice landraces of Northeast India.Plant cell reports · 2026Article
- Aluminum Genotoxicity in Plants, Mammals, and Unicellular Eukaryotes: The Underlying Mechanisms.International journal of molecular sciences · 2026Review
- Stimulatory Effect of Aluminum in Root Development ofInternational journal of molecular sciences · 2025Article
- Intrinsic and induced metabolic signatures underpin aluminum tolerance in bread wheat: a comparative metabolomics approach.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Article
- Mitigating aluminum toxicity and promoting plant resilience in acidic soil withFrontiers in plant science · 2024Article
- How Do Plants Cope with DNA Damage? A Concise Review on the DDR Pathway in Plants.International journal of molecular sciences · 2023Review
- Is it the end of TILLING era in plant science?Frontiers in plant science · 2023Review
- Fluoride mitigates aluminum-toxicity in barley: morpho-physiological responses and biochemical mechanisms.BMC plant biology · 2022Article
- Plant Cytogenetics in the Micronuclei Investigation-The Past, Current Status, and Perspectives.International journal of molecular sciences · 2022Review
- Aluminum in plant: Benefits, toxicity and tolerance mechanisms.Frontiers in plant science · 2022Review
- Enzymatic Activity and Its Relationship with Organic Matter Characterization and Ecotoxicity toSensors (Basel, Switzerland) · 2021Article
- Transcriptome Response to Cadmium Exposure in Barley (Frontiers in plant science · 2021Article
- The Role of Structural Maintenance of Chromosomes Complexes in Meiosis and Genome Maintenance: Translating Biomedical and Model Plant Research Into Crop Breeding Opportunities.Frontiers in plant science · 2021Review
- Comparative Inter- and IntraSpecies Transcriptomics Revealed Key Differential Pathways Associated With Aluminium Stress Tolerance in Lentil.Frontiers in plant science · 2021Article
- Aluminum or Low pH - Which Is the Bigger Enemy of Barley? Transcriptome Analysis of Barley Root Meristem Under Al and Low pH Stress.Frontiers in genetics · 2021Article
- Al-Tolerant Barley MutantInternational journal of molecular sciences · 2020Article
- Effect of the duration of use of aluminum cookware on its metal leachability and cytogenotoxicity in Allium cepa assay.Protoplasma · 2020Article
- Illumina sequencing revealed roles of microRNAs in different aluminum tolerance of two citrus species.Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Genetic control of some plant growth characteristics of bread wheat (Triticum aestivum L.) under aluminum stress.Genes & genomics · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Barley is one of the cereals that are most sensitive to aluminum (Al). Al in acid soils limits barley growth and development and, as a result, its productivity. The inhibition of root growth is a widely accepted indicator of Al stress. Al toxicity is affected by many factors including the culture medium, pH, Al concentration and the duration of the treatment. However, Al can act differently in different species and still Al toxicity in barley deserves study. Since the mechanism of Al toxicity is discussed we cytogenetically describe the effects of different doses of bioavailable Al on the barley nuclear genome-mitotic activity, cell cycle profile and DNA integrity. At the same time, we tested an established deep-water culture (DWC) hydroponics system and analyzed the effects of Al on the root system parameters using WinRHIZO software. We demonstrated the cytotoxic and genotoxic effect of Al in barley root cells. We showed that Al treatment significantly reduced the mitotic activity of the root tip cells and it also induced micronuclei and damaged nuclei. The DNA-damaging effect of Al was observed using the TUNEL test. We define the inhibitory influence of Al on DNA replication in barley. Analysis with the labelling and detection of 5-ethynyl-2'-deoxyuridin (EdU) showed that the treatment with Al significantly decreased the frequency of S phase cells. We also demonstrated that Al exposure led to changes in the cell cycle profile of barley root tips. The delay of cell divisions observed as increased frequency of cells in G2/M phase after Al treatment was reported using flow cytometry.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.