ArticleChembiochem : a European journal of chemical biology2018
Near-Infrared Light-Controlled Gene Expression and Protein Targeting in Neurons and Non-neuronal Cells.
Article in Chembiochem : a European journal of chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Environment signal dependent biocontainment systems for engineered organisms: Leveraging triggered responses and combinatorial systems.Synthetic and systems biotechnology · 2025Review
- Near-Infrared Optogenetic Module for Conditional Protein Splicing.Journal of molecular biology · 2023Article
- Directed differentiation of human iPSCs into mesenchymal lineages by optogenetic control of TGF-β signaling.Cell reports · 2023Article
- Plug-and-Display Photo-Switchable Systems on Plant Virus Nanoparticles.Biotech (Basel (Switzerland)) · 2022Article
- Optogenetic and Chemical Induction Systems for Regulation of Transgene Expression in Plants: Use in Basic and Applied Research.International journal of molecular sciences · 2022Review
- Nuclear Localization Signals for Optimization of Genetically Encoded Tools in Neurons.Frontiers in cell and developmental biology · 2022Article
- The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.Chemical reviews · 2021Review
- A guide to the optogenetic regulation of endogenous molecules.Nature methods · 2021Review
- Synthetic Biology: Emerging Concepts to Design and Advance Adeno-Associated Viral Vectors for Gene Therapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Toward Multiplexed Optogenetic Circuits.Frontiers in bioengineering and biotechnology · 2021Review
- Bacteriophytochromes - from informative model systems of phytochrome function to powerful tools in cell biology.Current opinion in structural biology · 2019Review
- Neurotrophin receptor tyrosine kinases regulated with near-infrared light.Nature communications · 2019Article
- Programming Bacteria With Light-Sensors and Applications in Synthetic Biology.Frontiers in microbiology · 2018Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
Abstract
Near-infrared (NIR) light-inducible binding of bacterial phytochrome BphP1 to its engineered partner, QPAS1, is used for optical protein regulation in mammalian cells. However, there are no data on the application of the BphP1-QPAS1 pair in cells derived from various mammalian tissues. Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons. We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS. In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types. The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells. The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.