Evidence map›Paper›PMID 29464982›Full record

ArticleCell cycle (Georgetown, Tex.)2018

RCC1 regulates inner centromeric composition in a Ran-independent fashion.

Michael Shaofei Zhang, Maiko Furuta, Alexei Arnaoutov, Mary Dasso

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell cycle (Georgetown, Tex.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Michael Shaofei Zhanga Division of Molecular and Cellular Biology , National Institute for Child Health and Human Development , National Institutes of Health , Bethesda , MD 20892.
Maiko Furutaa Division of Molecular and Cellular Biology , National Institute for Child Health and Human Development , National Institutes of Health , Bethesda , MD 20892.
Alexei Arnaoutova Division of Molecular and Cellular Biology , National Institute for Child Health and Human Development , National Institutes of Health , Bethesda , MD 20892.
Mary Dassoa Division of Molecular and Cellular Biology , National Institute for Child Health and Human Development , National Institutes of Health , Bethesda , MD 20892.
National Institutes of Health · US

Funding

Mitotic roles of the Nuclear Transport MachineryZIAHD008954 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI DASSO, MARY C. · 2015 to 2025
$25.3M
Regulation of Mitotic Kinetochores by the Ran GTPase Z01HD008740 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI DASSO, MARY C. · 2002 to 2008
$1.0M
PRENATAL AND INFANT HEALTH OF MEXICAN IMMIGRANTS IN U.S.F31HD008740 · NICHD · UNIVERSITY OF WISCONSIN MADISON · PI CEBALLOS, MIGUEL · 2001 to 2002
$45k
Intramural NIH HHS Z01 HD008740NICHD NIH HHS F31 HD008740
6 · The paper itself

Abstract

RCC1 associates to chromatin dynamically within mitosis and catalyzes Ran-GTP production. Exogenous RCC1 disrupts kinetochore structure in Xenopus egg extracts (XEEs), but the molecular basis of this disruption remains unknown. We have investigated this question, utilizing replicated chromosomes that possess paired sister kinetochores. We find that exogenous RCC1 evicts a specific subset of inner KT proteins including Shugoshin-1 (Sgo1) and the chromosome passenger complex (CPC). We generated RCC1 mutants that separate its enzymatic activity and chromatin binding. Strikingly, Sgo1 and CPC eviction depended only on RCC1's chromatin affinity but not its capacity to produce Ran-GTP. RCC1 similarly released Sgo1 and CPC from synthetic kinetochores assembled on CENP-A nucleosome arrays. Together, our findings indicate RCC1 regulates kinetochores at the metaphase-anaphase transition through Ran-GTP-independent displacement of Sgo1 and CPC.

Indexed as

AnimalsCell Cycle ProteinsCentromereCentromere Protein AChromatinGuanine Nucleotide Exchange FactorsHumansKinetochoresMitosisMutagenesis, Site-DirectedNuclear ProteinsOvumran GTP-Binding ProteinXenopus laevisXenopus ProteinsCell Cycle ProteinsCentromere Protein AChromatinGuanine Nucleotide Exchange FactorsNuclear Proteinsran GTP-Binding ProteinRCC1 protein, humanXenopus Proteinschromatin bindingchromosome passenger complexinner centromeremetaphase-anaphase transitionRanGEF independentRCC1Sgo1

Identifiers

PMID29464982
PMCPMC5969542
OpenAlexW2793458183

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.