ArticleScientific reports2018
Identification of stable reference genes for quantitative PCR in koalas.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Infection-Induced Telomere Length Variation: Insights into Pathogenesis of Koala Retrovirus.Viruses · 2025Article
- The relationships of viral and protozoal co-infections to Chlamydia pecorum infection and chlamydiosis outcomes in northern koalas (Phascolarctos cinereus).PLoS pathogens · 2025Article
- A novel multi-variate immunological approach, reveals immune variation associated with environmental conditions, and co-infection in the koala (Phascolarctos cinereus).Scientific reports · 2024Article
- Identifying the stability of housekeeping genes to be used for the quantitative real-time PCR normalization in retinal tissue of streptozotocin-induced diabetic rats.International journal of ophthalmology · 2024Article
- Screening and validation of the optimal panel of reference genes in colonic epithelium and relative cancer cell lines.Scientific reports · 2023Article
- Innovative GenExpA software for selecting suitable reference genes for reliable normalization of gene expression in melanoma.Scientific reports · 2022Article
- Identification of a miRNA-based non-invasive predictive biomarker of response to target therapy in BRAF-mutant melanoma.Theranostics · 2022Article
- Identification of suitable reference genes for mesenchymal stem cells from menstrual blood of women with endometriosis.Scientific reports · 2021Article
- Retroviral integrations contribute to elevated host cancer rates during germline invasion.Nature communications · 2021Article
- Selection and validation of reference genes for quantitative real-time PCR in the green microalgae Tetraselmis chui.PloS one · 2021Article
- Koala retrovirus diversity, transmissibility, and disease associations.Retrovirology · 2020Article
- Plasma Formate Is Greater in Fetal and Neonatal Rats Compared with Their Mothers.The Journal of nutrition · 2020Article
- Koala retrovirus viral load and disease burden in distinct northern and southern koala populations.Scientific reports · 2020Article
- Reference gene selection and myosin heavy chain (MyHC) isoform expression in muscle tissues of domestic yak (Bos grunniens).PloS one · 2020Article
- Selection and Validation of the Optimal Panel of Reference Genes for RT-qPCR Analysis in the Developing Rat Cartilage.Frontiers in genetics · 2020Article
- The Selection of Reference Genes for Quantitative Real-Time PCR in the Ashidan Yak Mammary Gland During Lactation and Dry Period.Animals : an open access journal from MDPI · 2019Article
- Selection of Reference Genes for Normalization of Real-Time PCR Data in Calliptamus italicus (Orthoptera: Acrididae) Under Different Temperature Conditions.Journal of insect science (Online) · 2019Article
- Identification of reliable reference genes for qRT-PCR in the ephemeral plant Arabidopsis pumila based on full-length transcriptome data.Scientific reports · 2019Article
- Selection of reference genes for the quantitative real-time PCR normalization of gene expression in Isatis indigotica fortune.BMC molecular biology · 2019Article
- miRNA Expression in Ovarian Cancer in Fresh Frozen, Formalin-fixed Paraffin-embedded and Plasma Samples.In vivo (Athens, Greece)Article
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To better understand host and immune response to diseases, gene expression studies require identification of reference genes with stable expression for accurate normalisation. This study describes the identification and testing of reference genes with stable expression profiles in koala lymph node tissues across two genetically distinct koala populations. From the 25 most stable genes identified in transcriptome analysis, 11 genes were selected for verification using reverse transcription quantitative PCR, in addition to the commonly used ACTB and GAPDH genes. The expression data were analysed using stable genes statistical software - geNorm, BestKeeper, NormFinder, the comparative ΔCt method and RefFinder. All 13 genes showed relative stability in expression in koala lymph node tissues, however Tmem97 and Hmg20a were identified as the most stable genes across the two koala populations.
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