Evidence map›Paper›PMID 29463827›Full record

ArticleScientific reports2018

Long non-coding RNA AC026166.2-001 inhibits cell proliferation and migration in laryngeal squamous cell carcinoma by regulating the miR-24-3p/p27 axis.

Zhisen Shen, Wenjuan Hao, Chongchang Zhou, Hongxia Deng, Dong Ye, Qun Li, Lexi Lin, Bing Cao, Junming Guo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 31 citations in OpenAlex.

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  19. Overexpression ofOncoTargets and therapy · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Zhisen ShenDepartment of Otorhinolaryngology and Head and Neck Surgery, the Affiliated Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China. szs7216@163.com.
Wenjuan HaoDepartment of Otorhinolaryngology and Head and Neck Surgery, the Affiliated Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China.
Chongchang ZhouDepartment of Otorhinolaryngology and Head and Neck Surgery, the Affiliated Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China.ORCID 0000-0002-8728-6819
Hongxia DengDepartment of Otorhinolaryngology and Head and Neck Surgery, the Affiliated Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China.
Dong YeDepartment of Otorhinolaryngology and Head and Neck Surgery, the Affiliated Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China.
Qun LiDepartment of Otorhinolaryngology and Head and Neck Surgery, the Affiliated Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China.
Lexi LinDepartment of Otorhinolaryngology and Head and Neck Surgery, the Affiliated Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China.
Bing CaoDepartment of Otorhinolaryngology and Head and Neck Surgery, the Affiliated Lihuili Hospital, Medical School of Ningbo University, Ningbo, 315040, China.
Junming GuoDepartment of Biochemistry and Molecular Biology, Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, Ningbo, 315211, China. guojunming@nbu.edu.cn.
Ningbo University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Long non-coding RNA (lncRNA) AC026166.2-001 was found to be down-regulated in laryngeal squamous cell carcinoma (LSCC) tissues and metastatic neck lymph nodes. Decreased AC026166.2-001 was associated with poorer prognosis and may act as a novel biomarker for LSCC patients. In this study, AC026166.2-001 was overexpressed by a lentivirus vector and down-regulated by a small interfering RNA (siRNA). The results of real-time cell analysis (RTCA) and a plate colony formation assay showed that AC026166.2-001 inhibited LSCC cell proliferation and the clone-forming capacity. Cell cycle distribution and related protein changes were measured by flow cytometry. AC026166.2-001 arrested the cell cycle at the G1 phase and induced apoptosis. In addition, AC026166.2-001 decreased cell migration as measured by wound healing assays and transwell migration assays. Moreover, luciferase reporter assay and Western blotting results suggested that AC026166.2-001 acts as a sponge of miR-24-3p and regulates the expression of p27 and cyclin D1. The in vivo results showed that AC026166.2-001 significantly suppressed the growth of LSCC xenografts and promoted apoptosis. We validated the molecular mechanisms underlying AC026166.2-001 in LSCC. This is the first report of AC026166.2-001 acting as a tumor suppressor in LSCC by regulating the miR-24-3p/p27 axis.

Indexed as

Cell MovementCell ProliferationApoptosisCarcinoma, Squamous CellCell CycleCell Line, TumorCytological TechniquesGene Expression ProfilingGene Expression RegulationHumansLaryngeal NeoplasmsMicroRNAsModels, BiologicalProliferating Cell Nuclear AntigenRNA, Long NoncodingMicroRNAsMIRN24 microRNA, humanp27 antigenProliferating Cell Nuclear AntigenRNA, Long Noncoding

Identifiers

PMID29463827
PMCPMC5820272
OpenAlexW2807250978

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.