ArticleScientific reports2018
True gene-targeting events by CRISPR/Cas-induced DSB repair of the PPO locus with an ectopically integrated repair template.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- Role of complementary-sense genes and intergenic region of beet curly top virus in intermolecular recombination frequency upon local infection in plants.Journal of virology · 2025Article
- Beyond a few bases: methods for large DNA insertion and gene targeting in plants.The Plant journal : for cell and molecular biology · 2025Review
- Enhancing tiny millets through genome editing: current status and future prospects.Molecular genetics and genomics : MGG · 2025Review
- Review
- Crop genome editing through tissue-culture-independent transformation methods.Frontiers in genome editing · 2024Review
- Applications and Prospects of CRISPR/Cas9-Mediated Base Editing in Plant Breeding.Current issues in molecular biology · 2023Review
- Genome Editing of Golden SNP-Carrying Lycopene Epsilon-Cyclase (International journal of molecular sciences · 2022Article
- An update on precision genome editing by homology-directed repair in plants.Plant physiology · 2022Article
- CRISPR-Cas gene editing technology and its application prospect in medicinal plants.Chinese medicine · 2022Review
- Review
- Gene targeting in polymerase theta-deficient Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2022Article
- Enhancing HR Frequency for Precise Genome Editing in Plants.Frontiers in plant science · 2022Article
- Gene Targeting Facilitated by Engineered Sequence-Specific Nucleases: Potential Applications for Crop Improvement.Plant & cell physiology · 2021Review
- CRISPR/dCas9-Based Systems: Mechanisms and Applications in Plant Sciences.Plants (Basel, Switzerland) · 2021Review
- Novel CRISPR/Cas applications in plants: from prime editing to chromosome engineering.Transgenic research · 2021Review
- Sequence modification on demand: search and replace tools for precise gene editing in plants.Transgenic research · 2021Review
- Efficient gene targeting in Nicotiana tabacum using CRISPR/SaCas9 and temperature tolerant LbCas12a.Plant biotechnology journal · 2021Article
- Induced Genetic Variations in Fruit Trees Using New Breeding Tools: Food Security and Climate Resilience.Plants (Basel, Switzerland) · 2021Review
- Targeted DNA insertion in plants.Proceedings of the National Academy of Sciences of the United States of America · 2021Review
- Applications of CRISPR-Cas in agriculture and plant biotechnology.Nature reviews. Molecular cell biology · 2020Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, several tools have become available for improved gene-targeting (GT) in plants. DNA breaks at specific sites activate local DNA repair and recombination, including recombination with ectopic sequences leading to GT. Large-scale transformation with the repair template can be avoided by pre-insertion of the repair template in the genome and liberation by sequence-specific nucleases (in planta GT procedure). Here, we tested whether release of the repair template was required for GT. Plants were transformed with constructs encoding a CRISPR/Cas nuclease with a recognition site in the endogenous PPO gene and a repair template harboring a 5' truncated PPO gene with two amino acid substitutions rendering the enzyme insensitive to the herbicide butafenacil. Selection resulted in so-called true GT events, repaired via homologous recombination at both ends of the gene and transmitted to the next generation. As the template was surrounded by geminiviral LIR sequences, we also tested whether replication of the template could be induced by crossing-in an integrated geminivirus REP gene. However, we could not find evidence for repair template replication by REP and we obtained similar numbers of GT events in these plants. Thus, GT is possible without any further processing of the pre-inserted repair template.
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