ArticlemBio2018
N-glycosylation of Viral E Protein Is the Determinant for Vector Midgut Invasion by Flaviviruses.
Article in mBio, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 66 citations in OpenAlex.
- m6A epigenetic modification of viral RNA is critical for the transmission cycle of mosquito-borne flaviviruses.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Article
- Therapeutic Targeting of Viral N-Glycosylation Modification: From Molecular Mechanisms to Clinical Application Prospects.Infectious diseases and therapy · 2025Review
- Variation in virion phosphatidylserine content drives differential GAS6 binding among closely related flaviviruses.Journal of virology · 2025Article
- The Role of Hematophagous Arthropods, Other than Mosquitoes and Ticks, in Arbovirus Transmission.Viruses · 2025Review
- Review
- Risk assessment of an Aedes flavivirus and its effect on pathogenic flavivirus replication in mosquitoes.Parasites & vectors · 2025Article
- Towards a Universal Translator: Decoding the PTMs That Regulate Orthoflavivirus Infection.Viruses · 2025Review
- Deficient of glycosylation site in the envelop protein attenuated Zika virus replication in mosquito cells.Frontiers in microbiology · 2025Article
- Evolution and adaptation of dengue virus in response to high-temperature passaging in mosquito cells.Virus evolution · 2025Article
- Evolution of Zika virus inVirus evolution · 2025Article
- Gut symbiont-derived sphingosine modulates vector competence in Aedes mosquitoes.Nature communications · 2024Article
- A Stable Reverse Genetics System of Zika Virus Based on a Self-Splicing Group II Intron.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A single amino acid substitution in the capsid protein of Zika virus contributes to a neurovirulent phenotype.Nature communications · 2023Article
- Article
- Article
- Morphologic and Genetic Characterization of Ilheus Virus, a Potential Emergent Flavivirus in the Americas.Viruses · 2023Article
- Suppression of flavivirus transmission from animal hosts to mosquitoes with a mosquito-delivered vaccine.Nature communications · 2022Article
- Pathogenicity and Structural Basis of Zika Variants with Glycan Loop Deletions in the Envelope Protein.Journal of virology · 2022Article
- Glycosylation of viral proteins: Implication in virus-host interaction and virulence.Virulence · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transmission of flaviviruses by hematophagous insects such as mosquitoes requires acquisition of the virus during blood feeding on the host, with midgut as the primary infection site. Here, we report that N-glycosylation of the E protein, which is conserved among most flaviviruses, is critical for the Zika virus (ZIKV) to invade the vector midgut by inhibiting the reactive oxygen species (ROS) pathway of the mosquito immune system. Our data further show that removal of the ZIKV E glycosylation site prevents mosquito infection by flaviviruses via the oral route, whereas there is no effect on infection by intrathoracic microinjection, which bypasses the midgut. Interestingly, the defect in infection of the mosquito midgut by the mutant virus through blood feeding is rescued by reduction of the ROS level by application of vitamin C, a well-known antioxidant. Therefore, our data demonstrate that ZIKV utilizes the glycosylation on the envelope to antagonize the vector immune defense during infection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.