Evidence map›Paper›PMID 29462992›Full record

ArticleMolecules (Basel, Switzerland)2018

New Bacterial Phytase through Metagenomic Prospection.

Nathálya Farias, Isabela Almeida, Carlos Meneses

Open access · goldAbstract read
In one paragraph

Article in Molecules (Basel, Switzerland), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
5.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Nathálya FariasGraduate Program in Agricultural Sciences, Universidade Estadual da Paraíba (UEPB), Campina Grande/PB 58429-500, Brazil. nathalyacfarias@gmail.com.
Isabela AlmeidaDepartment of Biology, Universidade Estadual da Paraíba (UEPB). isabelaalmeida42@outlook.com.ORCID 0000-0001-9995-782X
Carlos MenesesDepartment of Biology and Graduate Program in Agricultural Sciences, Universidade Estadual da Paraíba (UEPB), Campina Grande/PB 58429-500, Brazil. chmeneses@gmail.com.ORCID 0000-0001-8394-1305
Universidade Estadual da Paraíba · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alkaline phytases from uncultured microorganisms, which hydrolyze phytate to less phosphorylated myo-inositols and inorganic phosphate, have great potential as additives in agricultural industry. The development of metagenomics has stemmed from the ineluctable evidence that as-yet-uncultured microorganisms represent the vast majority of organisms in most environments on earth. In this study, a gene encoding a phytase was cloned from red rice crop residues and castor bean cake using a metagenomics strategy. The amino acid identity between this gene and its closest published counterparts is lower than 60%. The phytase was named PhyRC001 and was biochemically characterized. This recombinant protein showed activity on sodium phytate, indicating that PhyRC001 is a hydrolase enzyme. The enzymatic activity was optimal at a pH of 7.0 and at a temperature of 35 °C. β-propeller phytases possess great potential as feed additives because they are the only type of phytase with high activity at neutral pH. Therefore, to explore and exploit the underlying mechanism for β-propeller phytase functions could be of great benefit to biotechnology.

Indexed as

Metagenomics6-PhytaseAmino Acid SequenceBacteriaEnvironmentEnzyme StabilityGene LibraryGenes, BacterialHydrogen-Ion ConcentrationIonsMetalsModels, MolecularPhylogenyRecombinant ProteinsSequence Analysis, DNAStructural Homology, Protein6-PhytaseIonsMetalsRecombinant Proteinscrop residuesfunctional metagenomicsphytasic activity

Identifiers

PMID29462992
PMCPMC6017413
OpenAlexW2793004037

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.