ArticleThe New phytologist2018
A cytochrome P450 CYP71 enzyme expressed in Sorghum bicolor root hair cells participates in the biosynthesis of the benzoquinone allelochemical sorgoleone.
Article in The New phytologist, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
- Plant metabolic adaptation to nitrogen scarcity: the role of biological nitrification inhibitors.Essays in biochemistry · 2026Review
- Rhizome differentiation is associated with metabolic specialization and rhizosphere microbial assembly in Rheum officinale Baill.Plant cell reports · 2026Article
- Genome-wide characterization of the cytochrome P450 gene family in Solanum melongena and functional analysis of SmCYP82C1 under cold stress.BMC plant biology · 2025Article
- Comparative transcriptome analysis reveals key genes and signaling pathways mediated by salicylic acid in potato.BMC plant biology · 2025Article
- Enhancing Sorghum Growth: Influence of Arbuscular Mycorrhizal Fungi and Sorgoleone.Microorganisms · 2025Article
- BrCYP71 mutation resulted in stay-green in pak choi (Brassica rapa L. ssp. chinensis).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Phytotoxic Activity Analysis of 2-Methoxyphenol and 2,6-Di-Plants (Basel, Switzerland) · 2024Article
- Identifying genetic determinants of forage sorghum [Sorghum bicolor (Moench)] adaptation through GWAS.BMC plant biology · 2024Article
- Identification and Expression Analysis of Cytochrome P450 Genes Probably Involved in Triterpenoid Saponins Biosynthesis inInternational journal of molecular sciences · 2024Article
- Genome-wide identification of the CYP82 gene family in cucumber and functional characterization ofPeerJ · 2024Article
- Novel and holistic approaches are required to realize allelopathic potential for weed management.Ecology and evolution · 2023Review
- Phylotranscriptomic analyses reveal multiple whole-genome duplication events, the history of diversification and adaptations in the Araceae.Annals of botany · 2023Article
- Integrating Metabolomics and Transcriptomics to Unveil Atisine Biosynthesis inInternational journal of molecular sciences · 2022Article
- Article
- Variation on a theme: the structures and biosynthesis of specialized fatty acid natural products in plants.The Plant journal : for cell and molecular biology · 2022Review
- Article
- Progress and challenges in sorghum biotechnology, a multipurpose feedstock for the bioeconomy.Journal of experimental botany · 2022Review
- Comprehensive Analysis of Cytochrome P450 Monooxygenases Reveals Insight Into Their Role in Partial Resistance AgainstFrontiers in plant science · 2022Article
- Genome and transcriptome analysis to understand the role diversification of cytochrome P450 gene under excess nitrogen treatment.BMC plant biology · 2021Article
- Unraveling Sorghum Allelopathy in Agriculture: Concepts and Implications.Plants (Basel, Switzerland) · 2021Review
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Authors and funding
12 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sorgoleone, a major component of the hydrophobic root exudates of Sorghum spp., is probably responsible for many of the allelopathic properties attributed to members of this genus. Much of the biosynthetic pathway for this compound has been elucidated, with the exception of the enzyme responsible for the catalysis of the addition of two hydroxyl groups to the resorcinol ring. A library prepared from isolated Sorghum bicolor root hair cells was first mined for P450-like sequences, which were then analyzed by quantitative reverse transcription-polymerase chain reaction (RT-qPCR) to identify those preferentially expressed in root hairs. Full-length open reading frames for each candidate were generated, and then analyzed biochemically using both a yeast expression system and transient expression in Nicotiana benthamiana leaves. RNA interference (RNAi)-mediated repression in transgenic S. bicolor was used to confirm the roles of these candidates in the biosynthesis of sorgoleone in planta. A P450 enzyme, designated CYP71AM1, was found to be capable of catalyzing the formation of dihydrosorgoleone using 5-pentadecatrienyl resorcinol-3-methyl ether as substrate, as determined by gas chromatography-mass spectroscopy (GC-MS). RNAi-mediated repression of CYP71AM1 in S. bicolor resulted in decreased sorgoleone contents in multiple independent transformant events. Our results strongly suggest that CYP71AM1 participates in the biosynthetic pathway of the allelochemical sorgoleone.
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