ArticleNature chemistry2018
Evolving artificial metalloenzymes via random mutagenesis.
Article in Nature chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Artificial metalloenzymes in complex biological environments.Nature chemical biology · 2026Review
- Reprogramming NAD(P)Angewandte Chemie (International ed. in English) · 2026Article
- Intracellular assembly of artificial enzymes for cytoplasmic enantioselective Mannich reactions.Nature communications · 2026Article
- Chemical neighborhood exploration for substrate discovery in biocatalysis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Establishment of High-Throughput Screening Protocol Based on Isomerase UsingJournal of microbiology and biotechnology · 2025Article
- Artificial Gold Enzymes Using a Genetically Encoded Thiophenol-Based Noble-Metal-Binding Ligand.Angewandte Chemie (International ed. in English) · 2025Article
- Identification of Laccase Family ofInternational journal of molecular sciences · 2024Article
- Noncanonical Amino Acids: Bringing New-to-Nature Functionalities to Biocatalysis.Chemical reviews · 2024Review
- Computation-guided engineering of distal mutations in an artificial enzyme.Faraday discussions · 2024Article
- Enhanced Sequence-Activity Mapping and Evolution of Artificial Metalloenzymes by Active Learning.ACS central science · 2024Article
- Noncanonical Amino Acids in Biocatalysis.Chemical reviews · 2024Review
- Catch-and-Release: The Assembly, Immobilization, and Recycling of Redox-Reversible Artificial Metalloenzymes.ACS catalysis · 2024Article
- Non-Native Site-Selective Enzyme Catalysis.Chemical reviews · 2023Review
- Directed evolution of a wax ester synthase for production of fatty acid ethyl esters in Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2023Article
- Iridium(III) polypyridine artificial metalloenzymes with tunable photophysical properties: a new platform for visible light photocatalysis in aqueous solution.Dalton transactions (Cambridge, England : 2003) · 2023Article
- First and second sphere interactions accelerate non-nativeChemical communications (Cambridge, England) · 2023Article
- A primer to directed evolution: current methodologies and future directions.RSC chemical biology · 2023Review
- Evolutionary Engineering of a Cp*Rh(III) Complex-Linked Artificial Metalloenzyme with a Chimeric β-Barrel Protein Scaffold.Journal of the American Chemical Society · 2023Article
- Designing Artificial Metalloenzymes by Tuning of the Environment beyond the Primary Coordination Sphere.Chemical reviews · 2022Review
- Controlling the optical and catalytic properties of artificial metalloenzyme photocatalysts using chemogenetic engineering.Chemical science · 2022Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Random mutagenesis has the potential to optimize the efficiency and selectivity of protein catalysts without requiring detailed knowledge of protein structure; however, introducing synthetic metal cofactors complicates the expression and screening of enzyme libraries, and activity arising from free cofactor must be eliminated. Here we report an efficient platform to create and screen libraries of artificial metalloenzymes (ArMs) via random mutagenesis, which we use to evolve highly selective dirhodium cyclopropanases. Error-prone PCR and combinatorial codon mutagenesis enabled multiplexed analysis of random mutations, including at sites distal to the putative ArM active site that are difficult to identify using targeted mutagenesis approaches. Variants that exhibited significantly improved selectivity for each of the cyclopropane product enantiomers were identified, and higher activity than previously reported ArM cyclopropanases obtained via targeted mutagenesis was also observed. This improved selectivity carried over to other dirhodium-catalysed transformations, including N-H, S-H and Si-H insertion, demonstrating that ArMs evolved for one reaction can serve as starting points to evolve catalysts for others.
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