ArticleNature biomedical engineering2017
Non-destructive two-photon excited fluorescence imaging identifies early nodules in calcific aortic-valve disease.
Article in Nature biomedical engineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 41 citations in OpenAlex.
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- Elastic fiber alterations and calcifications in calcific uremic arteriolopathy.Scientific reports · 2023Article
- Application of the mineral-binding protein fetuin-A for the detection of calcified lesions.Theranostics · 2023Article
- Learned end-to-end high-resolution lensless fiber imaging towards real-time cancer diagnosis.Scientific reports · 2022Article
- Interdisciplinary Methods for Zoonotic Tissue Acellularization for Natural Heart Valve Substitute of Biomimetic Materials.Materials (Basel, Switzerland) · 2022Article
- Harnessing non-destructive 3D pathology.Nature biomedical engineering · 2021Review
- Label-Free Multiphoton Microscopy for the Detection and Monitoring of Calcific Aortic Valve Disease.Frontiers in cardiovascular medicine · 2021Review
- Label-free optical biomarkers detect early calcific aortic valve disease in a wild-type mouse model.BMC cardiovascular disorders · 2020Article
- Nonlinear optical microscopy is a novel tool for the analysis of cutaneous alterations in pseudoxanthoma elasticum.Lasers in medical science · 2020Article
- Label-free metabolic biomarkers for assessing valve interstitial cell calcific progression.Scientific reports · 2020Article
- Knockdown of CD44 expression decreases valve interstitial cell calcification in vitro.American journal of physiology. Heart and circulatory physiology · 2019Article
- Cardiovascular calcification: artificial intelligence and big data accelerate mechanistic discovery.Nature reviews. Cardiology · 2019Review
- Position Paper Computational Cardiology.IEEE journal of biomedical and health informatics · 2019Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Calcifications occur during the development of healthy bone, and at the onset of calcific aortic-valve disease (CAVD) and many other pathologies. Although the mechanisms regulating early calcium deposition are not fully understood, they may provide targets for new treatments and for early interventions. Here, we show that two-photon excited fluorescence (TPEF) can provide quantitative and sensitive readouts of calcific nodule formation, in particular in the context of CAVD. Specifically, by means of the decomposition of TPEF spectral images from excised human CAVD valves and from rat bone prior to and following demineralization, as well as from calcific nodules formed within engineered gels, we identified an endogenous fluorophore that correlates with the level of mineralization in the samples. We then developed a ratiometric imaging approach that provides a quantitative readout of the presence of mineral deposits in early calcifications. TPEF should enable non-destructive, high-resolution imaging of three-dimensional tissue specimens for the assessment of the presence of calcification.
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Registered trials
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