ArticleClinical epigenetics2018
Letter to the editor: blood processing and sample storage have negligible effects on methylation.
Article in Clinical epigenetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Guidelines for pre-analytical conditions for assessing the methylation of circulating cell-free DNA.Clinical epigenetics · 2021Pooled it
- Cumulative Stress Burden and Association With DNA Methylation in Ethiopian American Immigrants: Protocol for a Community-Engaged, Biopsychosocial Study.JMIR research protocols · 2026Article
- Alcohol consumption and DNA methylation: an epigenome-wide association study within the French E3N cohort.Clinical epigenetics · 2025Article
- Identification of diagnostic DNA methylation markers in the blood of Japanese Alzheimer's disease patients using methylation capture sequencing.Clinical epigenetics · 2025Article
- A Ge.F.I. Collaborative Study: Evaluating Reproducibility and Accuracy of a DNA-Methylation-Based Age-Predictive Assay for Routine Implementation in Forensic Casework.Electrophoresis · 2025Article
- Degradation of methylation signals in cryopreserved DNA.Clinical epigenetics · 2023Article
- Evaluation of Cell-Specific Epigenetic Age Acceleration in People With Multiple Sclerosis.Neurology · 2023Article
- Wild epigenetics: insights from epigenetic studies on natural populations.Proceedings. Biological sciences · 2022Review
- Ultralow amounts of DNA from long-term archived serum samples produce high-quality methylomes.Clinical epigenetics · 2021Article
- AHRR methylation in heavy smokers: associations with smoking, lung cancer risk, and lung cancer mortality.BMC cancer · 2020Article
- Epigenetic Biomarkers for Environmental Exposures and Personalized Breast Cancer Prevention.International journal of environmental research and public health · 2020Article
- DNA Methylation of Endoglin Pathway Genes in Pregnant Women With and Without Preeclampsia.Epigenetics insights · 2020Article
- Next-generation DNA methylation sequencing: loci and regions differentially methylated in adolescents with chronic postsurgical pain.Neurobiology of pain (Cambridge, Mass.)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation is a dynamic epigenetic mechanism. Researchers aiming to assess archived DNA samples are expressing concern about the effect of technical factors on methylation, as this may confound results. We reviewed recent reports examining this issue in blood samples and concluded that variation in collection, storage, and processing of blood DNA confers negligible effects on both global methylation and methylation status of specific genes. These results are concordant with studies that have investigated the effect of sample storage and processing on methylation in other tissues, such as tumour, sperm, and placenta samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.