ArticleCell systems2018
Molecular Time Sharing through Dynamic Pulsing in Single Cells.
Article in Cell systems, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Mixed positive and negative feedback loops drive diverse single-cell gene expression dynamics.Nature communications · 2026Article
- Growth rate-dependent mutations and phenotypic switching facilitate rapid adaptation.NPJ systems biology and applications · 2026Article
- Building resilience by cultivating difference: A role for noise in development.Current opinion in plant biology · 2025Review
- Engineering Sequestration-Based Biomolecular Classifiers with Shared Resources.ACS synthetic biology · 2024Article
- The alternative sigma factor SigN ofJournal of bacteriology · 2023Article
- The σB alternative sigma factor circuit modulates noise to generate different types of pulsing dynamics.PLoS computational biology · 2023Article
- Resonant learning in scale-free networks.PLoS computational biology · 2023Article
- Signal Transduction Network Principles Underlying Bacterial Collective Behaviors.Annual review of microbiology · 2022Review
- Phage-Encoded Sigma Factors Alter Bacterial Dormancy.mSphere · 2022Article
- Frequency modulation of a bacterial quorum sensing response.Nature communications · 2022Article
- Dynamic gene expression and growth underlie cell-to-cell heterogeneity inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Microfluidics for long-term single-cell time-lapse microscopy: Advances and applications.Frontiers in bioengineering and biotechnology · 2022Review
- Tunable phenotypic variability through an autoregulatory alternative sigma factor circuit.Molecular systems biology · 2021Article
- Microbial single-cell RNA sequencing by split-pool barcoding.Science (New York, N.Y.) · 2021Article
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- Functional roles of microbial cell-to-cell heterogeneity and emerging technologies for analysis and control.Current opinion in microbiology · 2020Review
- Stochastic pulsing of gene expression enables the generation of spatial patterns in Bacillus subtilis biofilms.Nature communications · 2020Article
- Isolating live cells after high-throughput, long-term, time-lapse microscopy.Nature methods · 2020Article
- Preexisting variation in DNA damage response predicts the fate of single mycobacteria under stress.The EMBO journal · 2019Article
- Stochastic antagonism between two proteins governs a bacterial cell fate switch.Science (New York, N.Y.) · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 5 institutions in 4 countries.
Funding
Abstract
In cells, specific regulators often compete for limited amounts of a core enzymatic resource. It is typically assumed that competition leads to partitioning of core enzyme molecules among regulators at constant levels. Alternatively, however, different regulatory species could time share, or take turns utilizing, the core resource. Using quantitative time-lapse microscopy, we analyzed sigma factor activity dynamics, and their competition for RNA polymerase, in individual Bacillus subtilis cells under energy stress. Multiple alternative sigma factors were activated in ∼1-hr pulses in stochastic and repetitive fashion. Pairwise analysis revealed that two sigma factors rarely pulse simultaneously and that some pairs are anti-correlated, indicating that RNAP utilization alternates among different sigma factors. Mathematical modeling revealed how stochastic time-sharing dynamics can emerge from pulse-generating sigma factor regulatory circuits actively competing for RNAP. Time sharing provides a mechanism for cells to dynamically control the distribution of cell states within a population. Since core molecular components are limiting in many other systems, time sharing may represent a general mode of regulation.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.