ArticleScientific reports2018
Validation of house-keeping genes for normalization of gene expression data during diurnal/circadian studies in rice by RT-qPCR.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Un-biased housekeeping gene panel selection for high-validity gene expression analysis.Scientific reports · 2022Pooled it
- Fine-tuning quantitative agronomic traits by manipulating gene copy number in rice.The New phytologist · 2026Article
- Chronic Night Shift Is More Detrimental Than Chronic Light at Night on Circadian Rhythmicity and Metabolic Health in a Female Diurnal Rodent.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Evaluation and validation of reference genes for RT-qPCR normalization in different sweet potato tissues.Scientific reports · 2025Article
- Identification of robust and abundant reference transcripts for EV mRNA cargo normalization.Extracellular vesicle · 2025Article
- Increased chloroplast area in the rice bundle sheath through cell-specific perturbation of brassinosteroid signaling.Plant physiology · 2025Article
- The immune receptor XA21 causes semi-male sterility and grain loss in rice.Frontiers in plant science · 2025Article
- Integrative transcriptome and metabolome evaluation of melanin biosynthesis inForestry research · 2025Article
- Transcriptomic analysis reveals cloquintocet-mexyl-inducible genes in hexaploid wheat (Triticum aestivum L.).PloS one · 2025Article
- Engineering quantitative stomatal trait variation and local adaptation potential by cis-regulatory editing.Plant biotechnology journal · 2024Article
- Genome-wide analysis of theFrontiers in plant science · 2024Article
- Paralog editing tunes rice stomatal density to maintain photosynthesis and improve drought tolerance.Plant physiology · 2023Article
- Conversion of sheath blight susceptible indica and japonica rice cultivars into moderately resistant through expression of antifungal β-1,3-glucanase transgene from Trichoderma spp.Transgenic research · 2022Article
- Selection and Validation of Candidate Reference Genes for Gene Expression Analysis by RT-qPCR inInternational journal of molecular sciences · 2021Article
- An optimized protocol for stepwise optimization of real-time RT-PCR analysis.Horticulture research · 2021Article
- Validation of suitable genes for normalization of diurnal gene expression studies in Chenopodium quinoa.PloS one · 2021Article
- Overexpression of ArabidopsisPlant signaling & behavior · 2020Article
- mRNA extraction of Xanthomonas fragariae in strawberry and validation of reference genes for the RT-qPCR for study of bacterial gene expression.Molecular biology reports · 2019Article
- Selection and Validation of Reference Genes for Gene Expression Studies by RT-PCR in Dalbergia odorifera.Scientific reports · 2019Article
- Selection of Reference Genes for qRT-PCR Analysis inMolecules (Basel, Switzerland) · 2018Article
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3 authors.
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Abstract
The circadian clock in plants is the intrinsic rhythmic expression of thousands of genes in a 24 h period, which is set by the day-night cycles in the environment. The study of the circadian clock often requires expression profiling of genes over a large number of samples for which RT-qPCR is invariably used. Reliability of the results depends largely on the house-keeping genes, which serve as control and thus should be chosen carefully to prevent erroneous results. In this study, ten house-keeping genes were chosen from rice for stability analysis with 48 tissue samples harvested from plants subjected to diurnal/circadian cycles. Although, all the genes were found to be stable, however, six of them showed cyclic expression patterns and caused major changes in the expression profiles of the target genes when used to normalize their expression data, thereby making them poor candidates for diurnal/circadian studies. In conclusion, reference genes need to be selected for diurnal/circadian studies with extra caution as more than 80% of transcriptome in plants undergoes cycling, which remains undetected by the gene stability assessment software and can severely affect the RT-qPCR based gene expression profiling. The geometric mean of two or more most stable reference genes is hence recommended for diurnal/circadian studies in plants.
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