ArticlePloS one2018
Rapid purification of giant lipid vesicles by microfiltration.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Preparation and biomedical applications of artificial cells.Materials today. Bio · 2023Review
- Measuring Encapsulation Efficiency in Cell-Mimicking Giant Unilamellar Vesicles.ACS synthetic biology · 2023Article
- A Microfluidic Platform for Sequential Assembly and Separation of Synthetic Cell Models.ACS synthetic biology · 2021Article
- Analysis of purification of charged giant vesicles in a buffer using their size distribution.The European physical journal. E, Soft matter · 2021Article
- A new purification technique to obtain specific size distribution of giant lipid vesicles using dual filtration.PloS one · 2021Article
- Low cost non-electromechanical technique for the purification of giant unilamellar vesicles.European biophysics journal : EBJ · 2019Article
- Towards the preparation of synthetic outer membrane vesicle models with micromolar affinity to wheat germ agglutinin using a dialkyl thioglycoside.Beilstein journal of organic chemistry · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Giant lipid vesicles (GVs) are emerging models for investigating the properties and reactivity of cell-like microcompartments, providing useful information about plausible protocellular structures in primitive times, as well as for the modern synthetic biology goal of constructing the first artificial cell from its reconstituted and partly modified components. Here we explore a novel methodology of GV purification by microfiltration under reduced pressure, operated by a simple apparatus. The method has been characterized in terms of flow rate, amount of lipid loss, quality of recovered GVs, and size distribution. A case study is reported to show the practicability of GV microfiltration. A clickable fluorescent probe was encapsulated inside GVs; more than 99.9% of the non-entrapped probe was easily and rapidly removed by multiple microfiltrations. This novel methodology is briefly discussed as a future tool for selection experiments on GV populations.
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Registered trials
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