ArticleClinical toxicology (Philadelphia, Pa.)2018
Revisiting the physiological effects of methylene blue as a treatment of cyanide intoxication.
Article in Clinical toxicology (Philadelphia, Pa.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 36 citations in OpenAlex.
- Extracellular flux analyses indicate low ATP yield and require refinement for accurate determination of maximal oxygen consumption rate.Scientific reports · 2026Article
- Cerebral Ischemia-Reperfusion Injury: Unraveling the Mitophagy-Oxidative Stress Axis for Neuroprotective Strategies.International journal of molecular sciences · 2026Review
- Methylene Blue Alleviates Inflammatory and Oxidative Lung Injury in a Rat Model of Feces-Induced Peritonitis.Medicina (Kaunas, Lithuania) · 2025Article
- Methylene blue mitigates lung injury in HCA rats by regulating macrophage pyroptosis via Nrf2/HO-1 and NLRP3 pathways.Biomolecules & biomedicine · 2025Article
- Redirecting Intermediary Metabolism to Counteract Cyanide Poisoning.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Review
- Spectroscopic Study of Methylene Blue Interaction with Coenzymes and its Effect on Tumor Metabolism.Sovremennye tekhnologii v meditsine · 2025Article
- Cyanide and Cyanogenic Compounds-Toxicity, Molecular Targets, and Therapeutic Agents.Biomolecules · 2024Review
- Methylene Blue-Mediated Photodynamic Therapy in Combination With Doxorubicin: A Novel Approach in the Treatment of HT-29 Colon Cancer Cells.Journal of lasers in medical sciences · 2024Article
- Adsorption of methylene blue dye from aqueous solution by a novel PVA/CMC/halloysite nanoclay bio composite: Characterization, kinetics, isotherm and antibacterial properties.Journal of environmental health science & engineering · 2020Article
- Antidotal effects of methylene blue against cyanide neurological toxicity: in vivo and in vitro studies.Annals of the New York Academy of Sciences · 2020Article
- The Delayed Neuroprotective Effect of Methylene Blue in Experimental Rat Brain Trauma.Antioxidants (Basel, Switzerland) · 2020Article
- Azure B as a novel cyanide antidote: Preclinical in-vivo studies.Toxicology reports · 2020Article
- Antidotal Effects of the Phenothiazine Chromophore Methylene Blue Following Cyanide Intoxication.Toxicological sciences : an official journal of the Society of Toxicology · 2019Article
- Methylene Blue Administration During and After Life-Threatening Intoxication by Hydrogen Sulfide: Efficacy Studies in Adult Sheep and Mechanisms of Action.Toxicological sciences : an official journal of the Society of Toxicology · 2019Article
- Methylene blue counteracts cyanide cardiotoxicity: cellular mechanisms.Journal of applied physiology (Bethesda, Md. : 1985) · 2018Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundAlthough methylene blue (MB) had long been proposed to counteract the effects of cyanide (CN) intoxication, research on its mechanisms of action and efficacy has been abandoned for decades. Recent studies on the benefits of MB in post-anoxic injuries have prompted us to reexamine the relevance of this historical observation.
methodsOur study was performed in adult male Sprague-Dawley rats and on HEK293T epithelial cells. First, the effects and toxicity of MB (0-80 mg/kg) on circulation and metabolism were established in four urethane-anesthetized rats. Then nine rats received a lethal infusion of a solution of KCN (0.75 mg/kg/min) and were treated by either saline or MB, at 20 mg/kg, a dose that we found to be innocuous in rat and to correspond to a dose of about 4 mg/kg in humans. MB was also administered 5 min after the end of a sub-lethal exposure to CN in a separate group of 10 rats. In addition, ATP/ADP ratio, ROS production, mitochondrial membrane potential (Δψm) and cellular O
resultsMethylene blue was found to be innocuous up to 50 mg/kg. KCN infusion (0.75 mg/kg/min) killed all animals within 7-8 min. MB (20 mg/kg) administered at the same time restored blood pressure, cardiac contractility and limited O
conclusionsThe present observations should make us consider the potential interest of MB in the treatment of CN intoxication. The mechanisms of the antidotal properties of MB cannot be accounted for by the creation of a cyanomethemoglobinemia, rather its protective effects appears to be related to the unique properties of this redox dye, which, depending on the dose, could directly oppose some of the consequences of the metabolic depression produced by CN at the cellular level.
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