ArticleChinese medicine2018
Authenticity analyses of Rhizoma Paridis using barcoding coupled with high resolution melting (Bar-HRM) analysis to control its quality for medicinal plant product.
Article in Chinese medicine, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed.
- Assessment of suitable cultivation area for Paris polyphylla var. chinensis and var. yunnanensis under anthropogenic disturbance based on ensemble modeling and germplasm identification.BMC plant biology · 2026Article
- Development of molecular markers for marker-assisted breeding and quality evaluation of Aconitum carmichaelii cultivars.BMC plant biology · 2025Article
- The genusHorticulture research · 2025Article
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- Successful development of molecular diagnostic technology combining mini-barcoding and high-resolution melting for traditional Chinese medicine agarwood species based on single-nucleotide polymorphism in the chloroplast genome.Frontiers in plant science · 2024Article
- Multiomics Reveals the Key Microorganisms and Metabolites in the Resistance to Root Rot Disease ofGenes · 2023Article
- DNA barcoding: an efficient technology to authenticate plant species of traditional Chinese medicine and recent advances.Chinese medicine · 2022Review
- Comparative analysis of medicinal plantFrontiers in plant science · 2022Article
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- The complete chloroplast genome sequence ofMitochondrial DNA. Part B, Resources · 2021Article
- The first complete chloroplast genome sequence ofMitochondrial DNA. Part B, Resources · 2020Article
- Quality evaluation based on color grading: quality discrimination of the Chinese medicine Corni Fructus by an E-eye.Scientific reports · 2019Article
- DNA Mini-Barcoding: A Derived Barcoding Method for Herbal Molecular Identification.Frontiers in plant science · 2019Review
- Paris saponin H suppresses human hepatocellular carcinoma (HCC) by inactivation of Wnt/β-catenin pathwayInternational journal of clinical and experimental pathology · 2019Article
- Sequence-Specific Detection ofFrontiers in plant science · 2018Article
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7 authors.
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Abstract
backgroundRhizoma Paridis (Chonglou) is a commonly used and precious traditional Chinese medicine.
methodsDNA barcoding coupled with high resolution melting analysis was applied in this research to distinguish Rhizoma Paridis from its adulteration. The internal transcribed spacer 2 (ITS2) barcode was selected for HRM analysis to produce standard melting profile of the selected species. DNA of the tested herbal medicines was isolated and their melting profiles were generated and compared with the standard melting profile of
resultsThe results indicate that the ITS2 molecular regions coupled with HRM analysis can effectively differentiate nine herbal species, including two authentic origins of Chonglou and their seven common adulterants. Ten herbal medicines labeled "Chonglou" obtained from a local market were collected and identified with our methods, and their sequence information was analyzed to validate the accuracy of HRM analysis.
conclusionsDNA barcoding coupled with HRM analysis is a accurate, reliable, rapid, cost-effective and robust tool, which could contribute to the quality control of Rhizoma Paridis in the supply chain of the natural health product industry (NHP).
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