ArticlePlant methods2018
An efficient modified method for plant leaf lipid extraction results in improved recovery of phosphatidic acid.
Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Ustilago maydis Phosphatidic Acid and Phosphatidylserine-Targeting Secreted Lipase Lip3 Is Involved in Pathogenesis.Molecular plant pathology · 2026Article
- Review
- Deciphering plant stress resilience in different millet crops: from multi-omics discoveries to predictive breeding for climate-resilient crop enhancement.Frontiers in plant science · 2026Review
- Sustainable enhancement of basil quality and resilience through biopriming with Pseudomonas JP0825.BMC plant biology · 2025Article
- Yield, Phytonutritional and Essential Mineral Element Profiles of Selected Aromatic Herbs: A Comparative Study of Hydroponics, Soilless and In-Soil Production Systems.Plants (Basel, Switzerland) · 2025Article
- Water stress enhances triacylglycerol accumulation via different mechanisms in wild-type and transgenic high-leaf oil tobacco.Plant physiology · 2025Article
- The biomass and health-enhancing qualities of lettuce are amplified through the inoculation of arbuscular mycorrhizal fungi.BMC plant biology · 2025Article
- Temporal proteome profiling of Botrytis cinerea reveals proteins involved in plant invasion and survival.Scientific reports · 2025Article
- Characterization of Archaea membrane lipids in radioactive springs using shotgun lipidomics.Folia microbiologica · 2025Article
- Synergistic Effect of Arbuscular Mycorrhizal Fungi and Germanium on the Growth, Nutritional Quality, and Health-Promoting Activities ofPlants (Basel, Switzerland) · 2024Article
- Hypoxia increases triacylglycerol levels and unsaturation in tomato roots.BMC plant biology · 2024Article
- Membrane protein provision controls prothylakoid biogenesis in tobacco etioplasts.The Plant cell · 2024Article
- Spatio-Temporal Study of Galactolipid Biosynthesis in Duckweed Using Mass Spectrometry Imaging and in vivo Isotope Labeling.Plant & cell physiology · 2024Article
- Inoculation withFrontiers in plant science · 2024Article
- Arabidopsis ACYL CARRIER PROTEIN4 and RHOMBOID LIKE10 act independently in chloroplast phosphatidate synthesis.Plant physiology · 2023Article
- Plastid Phosphatidylglycerol Homeostasis Is Required for Plant Growth and Metabolism inMetabolites · 2023Article
- Biological insights from multi-omics analysis strategies: Complex pleotropic effects associated with autophagy.Frontiers in plant science · 2023Article
- Lipid remodeling of contrasting maize (Frontiers in plant science · 2023Article
- Advances in Microbial NMR Metabolomics.Advances in experimental medicine and biology · 2023Article
- Lipid modulation contributes to heat stress adaptation in peanut.Frontiers in plant science · 2023Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLipidomics plays an important role in understanding plant adaptation to different stresses and improving our knowledge of the genes underlying lipid metabolism. Lipidomics involves lipid extraction, sample preparation, mass spectrometry analysis, and data interpretation. One of the practical challenges for large-scale lipidomics studies on plant leaves is the requirement of an efficient and rapid extraction method.
resultsA single-extraction method with a polar solvent mixture gives results comparable to a widely used, multi-extraction method when tested on both
conclusionsThe extract produced by this method can be used for analysis by mass spectrometry without a solvent evaporation step. The amount of lipid extracted, including phosphatidic acid, is comparable to widely used, more labor-intensive methods. The single-extraction protocol is less laborious, reducing the potential for human error.
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