ArticleJournal of physics D: Applied physics2017
Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane.
Article in Journal of physics D: Applied physics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 159 citations in OpenAlex.
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- A pectin-based formulation protects milk fat globule membranes in stored human milk.bioRxiv : the preprint server for biology · 2026Article
- Article
- The impact of charge on chlorpromazine interaction with lipid membranes.Journal of lipid research · 2026Article
- Disorder in Order-Related Membrane Biophysical Parameters: An In-Depth Analysis of Di-4-ANEPPDHQ Generalized Polarization.The journal of physical chemistry letters · 2026Article
- Quantification of cholesterol incorporation in giant unilamellar vesicles produced by a modified cDICE method.Soft matter · 2026Article
- Laurdan: Clarifying Photophysics and Advancing the Characterization of Extracellular Vesicles.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Lipid alterations in hereditary peripheral neuropathies: common mechanisms in disease heterogeneity?Molecular neurodegeneration · 2026Review
- Probing Guanidino Pendant or Bridged Groups in Cyclic Antimicrobial Peptides Derived from Temporin L: A Strategy to Improve Efficacy against Gram-Negative Bacteria.Journal of medicinal chemistry · 2025Article
- Examining the thermotropic properties of large circularized nanodiscs.Biochimica et biophysica acta. Biomembranes · 2025Article
- Comparative analysis of RT-qPCR, flow cytometry, and Di-4-ANEPPDHQ fluorescence for distinguishing macrophages phenotypes.Biochemistry and biophysics reports · 2025Article
- Temporizin-1 Meets the Membranes: Probing Membrane Inser-Tion and Disruption Mechanisms.Antibiotics (Basel, Switzerland) · 2025Article
- Boosting AMPs' Power: From Structural Engineering to Nanotechnology-Based Delivery.Molecules (Basel, Switzerland) · 2025Review
- Sterols in plant biologyCell surface (Amsterdam, Netherlands) · 2025Review
- Unremodeled GPI-anchored proteins at the plasma membrane trigger aberrant endocytosis.Life science alliance · 2025Article
- Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques?Membranes · 2025Review
- Imaging Plant Lipids with Fluorescent Reporters.Plants (Basel, Switzerland) · 2024Review
- VISION - an open-source software for automated multi-dimensional image analysis of cellular biophysics.Journal of cell science · 2024Article
- Synergistic effects of oxidative and acid stress on bacterial membranes of Escherichia coli and Staphylococcus simulans.Communications biology · 2024Article
18 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Lipid packing is a crucial feature of cellular membranes. Quantitative analysis of membrane lipid packing can be achieved using polarity sensitive probes whose emission spectrum depends on the lipid packing. However, detailed insights into the exact mechanisms that cause the changes in the spectra are necessary to interpret experimental fluorescence emission data correctly. Here, we analysed frequently used polarity sensitive probes, Laurdan and di-4-ANEPPDHQ, to test whether the underlying physical mechanisms of their spectral changes are the same and, thus, whether they report on the same physico-chemical properties of the cell membrane. Steady-state spectra as well as time-resolved emission spectra of the probes in solvents and model membranes revealed that they probe different properties of the lipid membrane. Our findings are important for the application of these dyes in cell biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.