ArticleScientific reports2018
Identification of G-quadruplex clusters by high-throughput sequencing of whole-genome amplified products with a G-quadruplex ligand.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- An updated overview of experimental and computational approaches to identify non-canonical DNA/RNA structures with emphasis on G-quadruplexes and R-loops.Briefings in bioinformatics · 2022Pooled it
- Kinetoplast-Directed Therapies: A Selective Mitochondrial Approach to Combat Leishmaniasis.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Archaeal G-quadruplexes: a novel model for understanding unusual DNA/RNA structures across the tree of life.Nucleic acids research · 2026Article
- Review
- Investigating G-quadruplex structures inHeliyon · 2024Article
- Crosstalk between G-quadruplex and ROS.Cell death & disease · 2023Review
- Starfish infers signatures of complex genomic rearrangements across human cancers.Nature cancer · 2022Article
- Quantitative detection of CpG methylation level on G-quadruplex and i-motif-forming DNA by recombinase polymerase amplification.Analytical and bioanalytical chemistry · 2022Article
- G-quadruplexes in helminth parasites.Nucleic acids research · 2022Article
- Ubiquitin-mediated DNA damage response is synthetic lethal with G-quadruplex stabilizer CX-5461.Scientific reports · 2021Article
- Article
- Effects of CpG methylation on the thermal stability ofBBA advances · 2021Article
- Perspectives for Applying G-Quadruplex Structures in Neurobiology and Neuropharmacology.International journal of molecular sciences · 2019Review
- The Presence and Localization of G-Quadruplex Forming Sequences in the Domain of Bacteria.Molecules (Basel, Switzerland) · 2019Article
- CpG Methylation Changes G-Quadruplex Structures Derived from Gene Promoters and Interaction with VEGF and SP1.Molecules (Basel, Switzerland) · 2018Article
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Authors and funding
11 authors.
Funding
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Abstract
G-quadruplex (G4) is a DNA secondary structure that has been found to play regulatory roles in the genome. The identification of G4-forming sequences is important to study the specific structure-function relationships of such regions. In the present study, we developed a method for identification of G4 clusters on genomic DNA by high-throughput sequencing of genomic DNA amplified via whole-genome amplification (WGA) in the presence of a G4 ligand. The G4 ligand specifically bound to G4 structures on genomic DNA; thus, DNA polymerase was arrested on the G4 structures stabilised by G4 ligand. We utilised the telomestatin derivative L1H1-7OTD as a G4 ligand and demonstrated that the efficiency of amplification of the G4 cluster regions was lower than that of the non-G4-forming regions. By high-throughput sequencing of the WGA products, 9,651 G4 clusters were identified on human genomic DNA. Among these clusters, 3,766 G4 clusters contained at least one transcriptional start site, suggesting that genes are regulated by G4 clusters rather than by one G4 structure.
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