ArticleNature communications2018
Mapping molecular assemblies with fluorescence microscopy and object-based spatial statistics.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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Who cites it
57 citing papers in PubMed.
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- Targeting myoferlin in ER/Golgi vesicle trafficking reprograms pancreatic cancer-associated fibroblasts.The EMBO journal · 2025Article
- A Molecular Recruitment Colocalization Platform for Visualizing Multi-Protein Interactions and Engineering Biomolecular Condensates in Living Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The scaffolding and activation of NLRP3 on acidic vesicles depends on their biophysical properties and is independent of intermediate filaments.Molecular biology of the cell · 2025Article
- Identifying intermolecular interactions in single-molecule localization microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Statistical analysis of spatial patterns in tumor microenvironment images.Nature communications · 2025Article
- Ubiquitin-mediated recruitment of the ATG9A-ATG2 lipid transfer complex drives clearance of phosphorylated p62 aggregates.Molecular biology of the cell · 2025Article
- Super-resolution microscopy and deep learning methods: what can they bring to neuroscience: from neuron to 3D spine segmentation.Frontiers in neuroinformatics · 2025Review
- Phosphorylation of Optineurin by protein kinase D regulates Parkin-dependent mitophagy.iScience · 2024Article
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- CoPixie, a novel algorithm for single-particle track colocalization, enables efficient quantification of telomerase dynamics at telomeres.Nucleic acids research · 2024Article
- Intracellular spatial transcriptomic analysis toolkit (InSTAnT).Nature communications · 2024Article
- AMPK regulates phagophore-to-autophagosome maturation.The Journal of cell biology · 2024Article
- Microglial TNFα controls daily changes in synaptic GABAARs and sleep slow waves.The Journal of cell biology · 2024Article
- A modular framework for multi-scale tissue imaging and neuronal segmentation.Nature communications · 2024Article
- Molecular mapping of neuronal architecture using STORM microscopy and new fluorescent probes for SMLM imaging.Neurophotonics · 2024Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elucidating protein functions and molecular organisation requires to localise precisely single or aggregated molecules and analyse their spatial distributions. We develop a statistical method SODA (Statistical Object Distance Analysis) that uses either micro- or nanoscopy to significantly improve on standard co-localisation techniques. Our method considers cellular geometry and densities of molecules to provide statistical maps of isolated and associated (coupled) molecules. We use SODA with three-colour structured-illumination microscopy (SIM) images of hippocampal neurons, and statistically characterise spatial organisation of thousands of synapses. We show that presynaptic synapsin is arranged in asymmetric triangle with the 2 postsynaptic markers homer and PSD95, indicating a deeper localisation of homer. We then determine stoichiometry and distance between localisations of two synaptic vesicle proteins with 3D-STORM. These findings give insights into the protein organisation at the synapse, and prove the efficiency of SODA to quantitatively assess the geometry of molecular assemblies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.